Title : ( Molecular cloning and the expression of the Na+/H+antiporter in the monocot halophyte Leptochloa fusca (L.) Kunth )
Authors: bahman panahi , Farajollah Shahriari Ahmadi , Mohammad Zare Mehrjerdi , Nasrin Moshtaghi ,
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Abstract
The plant vacuolar Na+ /H+ antiporter plays an important role in salt tolerance. A vacuolar Na+ /H+ cDNA (LfNHX1) gene was cloned from Leptochloa fusca(L.) Kunth, a halophyte forage grass, by cDNA and RACE methods using degenerate primers. The results show that the LfNHX sequence contains 2452 nucleotides and an open-reading frame of1620 nucleotides that encode a protein of 540 amino acids with a deduced molecular mass of 59.8 kDa. The deduced amino acid contains the consensus amiloride-binding domain. The effects of NaCl stress on the Na+ / H+ gene expression were analysed using Real Time PCR. The analysis showed that the expression level of LfNHX1 increases in response to different levels of NaCl, indicating that V-Na+ /H+ plays an important role in salt tolerance in this plant
Keywords
, NHX, vacuolar Na+ /H+ antiporter; ORF, open reading frame; RACE, rapid amplification of cDNA ends; TM, transmembrane; UTR, untranslated region.
@article{paperid:1035703,
author = {Panahi, Bahman and Shahriari Ahmadi, Farajollah and Zare Mehrjerdi, Mohammad and Moshtaghi, Nasrin},
title = {Molecular cloning and the expression of the Na+/H+antiporter in the monocot halophyte Leptochloa fusca (L.) Kunth},
journal = {NJAS - Wageningen Journal of Life Sciences},
year = {2013},
volume = {65},
number = {2},
month = {January},
issn = {1573-5214},
pages = {87--93},
numpages = {6},
keywords = {NHX; vacuolar Na+ /H+ antiporter; ORF; open reading frame; RACE;
rapid amplification of cDNA ends; TM; transmembrane; UTR; untranslated region.},
}
%0 Journal Article
%T Molecular cloning and the expression of the Na+/H+antiporter in the monocot halophyte Leptochloa fusca (L.) Kunth
%A Panahi, Bahman
%A Shahriari Ahmadi, Farajollah
%A Zare Mehrjerdi, Mohammad
%A Moshtaghi, Nasrin
%J NJAS - Wageningen Journal of Life Sciences
%@ 1573-5214
%D 2013
