The aim of this study was investigation of various levels of vitamin E in Tris extender on semen characteristics of Ghezel rams in pre and post freezing conditions. Semen was collected from six healthy and mature rams with average 55±5 Kg body weight by electro ejaculator. High quality samples were mixed and diluted in Tris extender after apply different concentration of vitamin E. Treatments were various levels of vitamin E (0, 1, 2, 3, 4 and 5 m/M). The semen aliquots were chilled and preserved at 5°C and their quality was evaluated in pre freezing, and then cooling semen samples entered into 0.5 ml of straws. At first, straws were frozen upper vapor liquid nitrogen,and then kept in -196ºC. After 10 days straws were thawed and some characteristics of spermatozoa were examined. Analysis of data was carried based on completely randomized design. Results of this study showed that the effect of vitamin E on characteristics of viability, motility, progressive motility and normal of spermatozoa were significant in pre and post freezing conditions (P<0.05), and the highest percentages of this treats were obtained in treatment of 2m/M vitamin E. In conclusion for storage long term of Ghezel rams spermatozoa, using 2mg/ml of vitamin E in semen extender is recommend.

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