Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, ( ISI ), Volume (122), No (1), Year (2013-11) , Pages (118-129)

Title : ( Synthesis, characterization and the interaction of some new water-soluble metal Schiff base complexes with human serum albumin )

Authors: Mozaffar Asadi , Zahra Asadi , Somaye Barzegar Sadi , Leila Zarei , Fatemeh Moosavi , Zahra Amirghofran ,

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Abstract

Some new water-soluble Schiff base complexes of Na2[M(L)(H2O)n]; (M = Zn, Cu, Ni, Mn) with a new water-soluble Schiff base ligand where L denotes an asymmetric N2O2 Schiff base ligands; N,N’-bis(5-sulfosalicyliden)-3,4-diaminobenzophenone (5-SO3-3,4-salbenz) were synthesized and characterized. The formation constants of the water soluble Schiff base complexes were calculated by Ketelaar\\\\\\\'s equation. The theoretical molecular structure for the complexes was computed by using the HF method and the 6-311G basis set. The mechanism of binding of Na2[M(L)(H2O)n] with human serum albumin (HSA) was studied by fluorescence spectroscopic technique. The results of fluorescence titration showed that the intrinsic fluorescence of HSA was quenched by the complexes; which was rationalized in terms of the dynamic quenching mechanism. The values of Stern-Volmer constants, quenching rate constants, binding constants, binding sites and average aggregation number of HSA have been determined. The thermodynamic parameters, were calculated by van’t Hoff equation, indicate that the binding is entropy driven and enthalpically disfavored. Based on the Förster theory of nonradiation energy transfer, the efficiency of energy transfer and the distance between the donor (Trp residues) and the acceptor (complex) were obtained. Finally, the growth inhibitory effects of the complexes toward the K562 cancer cell line were measured.

Keywords

, Schiff base, human serum albumin, fluorescence
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@article{paperid:1037316,
author = {Mozaffar Asadi and Zahra Asadi and Somaye Barzegar Sadi and Leila Zarei and Moosavi, Fatemeh and Zahra Amirghofran},
title = {Synthesis, characterization and the interaction of some new water-soluble metal Schiff base complexes with human serum albumin},
journal = {Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy},
year = {2013},
volume = {122},
number = {1},
month = {November},
issn = {1386-1425},
pages = {118--129},
numpages = {11},
keywords = {Schiff base; human serum albumin; fluorescence},
}

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%0 Journal Article
%T Synthesis, characterization and the interaction of some new water-soluble metal Schiff base complexes with human serum albumin
%A Mozaffar Asadi
%A Zahra Asadi
%A Somaye Barzegar Sadi
%A Leila Zarei
%A Moosavi, Fatemeh
%A Zahra Amirghofran
%J Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
%@ 1386-1425
%D 2013

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