Infectious keratoconjunctivitis(IKC) is a painful, highly contagious ocular disease in sheep and goats. This study was carried out to identification and characterization of causative agent of ocular disease in a sheep flock consisting of 300 ewes in mashhad/Iran. Several ocular swabs were taken from affected animals. The samples were pooled and processed in labopratory for isolation of suspecious agent. Following inoculation of the pooled sample in PPLO broth and agar, turbidity and growth of colonies were observed in them respectively. Sequencing of the 1013 bp PCR product of 16S rDNA gene revealed that the causative agent of the outbreak has 100% sequence identity to Mycoplasma conjunctivae. Based on our Knowledge this is the first documented report of isolation and molecular characterization of M. conjunctivae in Iran.

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