Cell Biology International, ( ISI ), Volume (38), Year (2014-1) , Pages (72-84)

Title : ( Chitosan-based injectable hydrogel as a promising in situ forming scaffold for cartilage tissue engineering )

Authors: Hojjat Naderi Meshkin , Kristin Andreas , Maryam Moghaddam Matin , Michael Sittinger , Hamid reza Bidkhori , Naghmeh Ahmadiankia , Ahmad Reza Bahrami , Jochen Ringe ,

Citation: BibTeX | EndNote

Chitosan-beta glycerophosphate-hydroxyethyl cellulose (CH-GP-HEC) is a biocompatible and biodegradable scaffold exhibiting a sol- gel transition at 37C. Chondrogenic factors or mesenchymal stem cells (MSCs) can be included in the CH-GP-HEC, and injected into the site of injury to fill the cartilage tissue defects with minimal invasion and pain. The possible impact ofthe injectable CH-GP-HEC on the viability of the encapsulated MSCs was assessed by propidium iodide-fluorescein diacetate staining. Proliferation of the human and rat MSCs was also determined by MTS assayon days 0, 7, 14 and 28 after encapsulation. To investigate the potential application of CH-GP-HEC as a drug delivery device, the in vitro release profile of insulin was quantified by QuantiPro-BCATM protein assay. Chondrogenic differentiation capacity of the encapsulated human MSCs (hMSCs) was also determined after induction of differentiation with transforming growth factor b3. MSCs have very good survival and proliferative rates within CH-GP-HEC hydrogel during the 28-day investigation. A sustained release of insulin occurred over 8 days. The CH-GP-HEC hydrogel also provided suitable conditions for chondrogenic differentiation of the encapsulated hMSCs. In conclusion, the high potential of CH-GP-HEC as an injectable hydrogel for cartilage tissue engineering is emphasised

Keywords

cartilage tissue engineering; chitosan injectable hydrogel; chondrogenesis; in vitro release profile; mesenchymal stem
برای دانلود از شناسه و رمز عبور پرتال پویا استفاده کنید.

@article{paperid:1038383,
author = {Naderi Meshkin, Hojjat and Kristin Andreas and Moghaddam Matin, Maryam and Michael Sittinger and Bidkhori, Hamid Reza and Naghmeh Ahmadiankia and Bahrami, Ahmad Reza and Jochen Ringe},
title = {Chitosan-based injectable hydrogel as a promising in situ forming scaffold for cartilage tissue engineering},
journal = {Cell Biology International},
year = {2014},
volume = {38},
month = {January},
issn = {1065-6995},
pages = {72--84},
numpages = {12},
keywords = {cartilage tissue engineering; chitosan injectable hydrogel; chondrogenesis; in vitro release profile; mesenchymal stem cells},
}

[Download]

%0 Journal Article
%T Chitosan-based injectable hydrogel as a promising in situ forming scaffold for cartilage tissue engineering
%A Naderi Meshkin, Hojjat
%A Kristin Andreas
%A Moghaddam Matin, Maryam
%A Michael Sittinger
%A Bidkhori, Hamid Reza
%A Naghmeh Ahmadiankia
%A Bahrami, Ahmad Reza
%A Jochen Ringe
%J Cell Biology International
%@ 1065-6995
%D 2014

[Download]