Journal of Sciences, Islamic Republic of Iran, ( ISI ), Volume (15), No (4), Year (2004-10) , Pages (303-309)

Title : ( Purification and Characterization of an Antibacterial, Antifungal and Non Hemolytic Peptide from Rana Ridibunda )

Authors: Ahmad Asoodeh , H. Naderi-Manesh , M. Mirshahi , B. Ranjbar ,

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Amphibians have a large variety of antimicrobial peptides that serve as natural innate barriers limiting microbial infection or, in some instances, act as an integral component in response to inflammation or microbial infection. A novel peptide with antibacterial effects and without hemolytic activity was purified from skin secretions of Rana ridibunda by multisteps cation-exchange FPLC, reversedphase HPLC and was called Ridibundin 1. Circular dichroism spectra revealed that this peptide strongly prefers to form an amphipathic α-helical structure in the presence of 50% trifluoroethanol. Acting as wide-spectrum microbicides against a variety of bacteria, Ridibundin 1 also shows no hemolytic activity on erythrocyte. These properties reveal its unique characteristics and potential therapeutic application.

Keywords

Antibacterial peptide; Hemolysis; Rana
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@article{paperid:1039831,
author = {Asoodeh, Ahmad and H. Naderi-Manesh and M. Mirshahi and B. Ranjbar},
title = {Purification and Characterization of an Antibacterial, Antifungal and Non Hemolytic Peptide from Rana Ridibunda},
journal = {Journal of Sciences, Islamic Republic of Iran},
year = {2004},
volume = {15},
number = {4},
month = {October},
issn = {1016-1104},
pages = {303--309},
numpages = {6},
keywords = {Antibacterial peptide; Hemolysis; Rana ridibunda},
}

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%0 Journal Article
%T Purification and Characterization of an Antibacterial, Antifungal and Non Hemolytic Peptide from Rana Ridibunda
%A Asoodeh, Ahmad
%A H. Naderi-Manesh
%A M. Mirshahi
%A B. Ranjbar
%J Journal of Sciences, Islamic Republic of Iran
%@ 1016-1104
%D 2004

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