Objectives:There are more than 120 named Mycoplasma species and more than 20 species are known to infect avian hosts. Mycoplasma columborale, Mycoplasma columbinumand M. columbinasale are recognized pigeon species of mycoplasma. They have been recovered from pigeons in Euroupean countries but there is no reports in Asian countries yet. Materials & Methods: Oral swab from choana of presented diseased laughing bird with respiratory signs were collected in thoroughly sterile conditions. Sample was cultured for Mycoplasma species. Extracted DNA from Mycoplasma positive sample wasanalyzed using genus and species specific PCRs.The PCR product was sequenced in two ways by using the same forward and reverse primers and the sequence of the product was compared to the 16S rRNA gene data of Mycoplasma spp. deposit GeneBank, using Blast , nucleotides alignment and phylogenetic tree softwares. Results & Conclusion: The results of this study indicate that species of mycoplasma isolated from laughing dove Streptopeliasenegalensisermanni, wasM. columborale.

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