Background: The Iranian medicinal plants, such as Satureja bachtiarica have been utilized as traditional medicines by the indigenous people of Chaharmahal and Bakhtiari in Iran. According to biologically active compounds and traditional use of the Satureja bachtiarica, seem that this plant has significant antimicrobial effects. Materials and Methods: In this experimental study, Satureja bachtiarica after extraction with watery, ethanol 96%, methanol 96% and 20% glycerin antimicrobial effect of extract were determined by “screening antimicrobial activity” and “disk agar diffusion test” in 10, 20, 30 and 40 mg/mL concentration of the extract against Streptococcus pyogenes PTCC 1447, Pseudomonas aeruginosa PTCC 1310 and Staphylococcus epidermidis PTCC 1435. Statistical analysis was carried out by analysis of variance (ANOVA). Results: The results showed that all extracts were quite effective in 2 mg/mL concentration on S. pyogenes and S. epidermidis and were prevented from growth them on medium, while extracts have no certain antimicrobial effect on P. aeruginosa. In “disk agar diffusion method”, 10, 20, 30 and 40 mg/mL aqueous, ethanol 96%, methanol 96% and 20% glycerin extract concentrations, was inhibited effect on S. pyogenes and S. epidermidis, but 40 mg/mL aqueous and 30 and 40 mg/mL ethanol, methanol and glycerin extract concentrations, has inhibited effect on P. aeruginosa prevent them growing. The results indicate that alcoholic and aqueous extracts of S. bachtiarica have the greatest effect on gram positive bacterium S. pyogenes. Conclusion: As a result aqueous and alcoholic extracts of S. bachtiarica, have been strong antimicrobial activity against many food pathogen bacteria.

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