The purpose of this study was to investigate the effect of RAST anaerobic test on CPK and LDH enzymes in active and non-active females. This research was performed through Quasi experimental method. Statistical sample included 10 athlete and 10 non athlete female students from ALzahra University who were selected randomly. Blood samples were collected from subjects be fore and immediately after exercise. Physical activity consisted of performance of the anaerobic RAST test with maximal effort of 85%- 95% subjects ́ heart rate reserved. At the end data was analyzed through mixed factorial and multivariate test. The findings of the study indicated: There was a significant increase in levels of LDH and CPK enzyme between active and non–active female students after exercise.There was no significant difference between active and inactive female students in terms of CK and LDH enzymes activity.

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