Journal of Cereal Science, ( ISI ), Volume (60), No (1), Year (2014-6) , Pages (92-98)

Title : ( Potential angiotensin I converting enzyme inhibitory peptides from gluten hydrolysate: Biochemical characterization and molecular docking study )

Authors: Ahmad Asoodeh , Leyla Haghighi , Jamashidkhan Chamani , Mohamad Amin Ansari-Ogholbeyk , zahra mojallal taba tabaei , Milad Lagzian ,

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The present study was carried out to characterize ACE inhibitory peptides which are released from the trypsin hydrolysate of wheat gluten protein. In silico proteolitic digestion of a high molecular weight glutenin subunit was performed. Among the resultant fragments, four peptides were selected for chemical synthesis based on the chemoinformatics studies and docking properties. The ACE inhibitory activity and kinetic parameters of the most important peptides were determined. Molecular docking simulation was also performed to predict the sites on ACE in which these peptides bind and displayed inhibition mechanisms. Two peptide sequences of IPALLKR (P4) and AQQLAAQLPAMCR (P6) showed higher ACE inhibitory activity among peptide collection. The IC50 values of P6 and P4 were 43  1.3 mM and 68  2.8 mM, respectively. P6 peptide was proved to be a more potent ACE inhibitor than P4 peptide. Lineweaver-Burk plots revealed that P6 and P4 behaved as non-competitive and competitive ACE inhibitors, respectively. The simulations showed that P4 bound to the active site region. Conversely, P6 bound to the N-terminus entrance of substrate tunnel and obstructed the substrate access into the catalytic site. Overall, the results showed that these peptides would be considered as a model for discovering new bio-compatible ACE inhibitors.

Keywords

, Gluten, ACE-inhibitory peptide, Docking
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@article{paperid:1041757,
author = {Asoodeh, Ahmad and Leyla Haghighi and Jamashidkhan Chamani and Mohamad Amin Ansari-Ogholbeyk and Mojallal Taba Tabaei, Zahra and Lagzian, Milad},
title = {Potential angiotensin I converting enzyme inhibitory peptides from gluten hydrolysate: Biochemical characterization and molecular docking study},
journal = {Journal of Cereal Science},
year = {2014},
volume = {60},
number = {1},
month = {June},
issn = {0733-5210},
pages = {92--98},
numpages = {6},
keywords = {Gluten; ACE-inhibitory peptide; Docking simulation},
}

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%0 Journal Article
%T Potential angiotensin I converting enzyme inhibitory peptides from gluten hydrolysate: Biochemical characterization and molecular docking study
%A Asoodeh, Ahmad
%A Leyla Haghighi
%A Jamashidkhan Chamani
%A Mohamad Amin Ansari-Ogholbeyk
%A Mojallal Taba Tabaei, Zahra
%A Lagzian, Milad
%J Journal of Cereal Science
%@ 0733-5210
%D 2014

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