Journal of Sciences, Islamic Republic of Iran, ( ISI ), Volume (25), No (1), Year (2014-6) , Pages (5-12)

Title : ( Biochemical Characterization of A Novel Thermophilic Esterase Isolated from Shewanella sp F88 )

Authors: Ahmad Asoodeh , Adel Ghorani Azam , HADI ZARE ZARDINI ,

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The main objective of this study was to purify and characterize an esterase from Shewanella sp F88. The enzyme was purified 41-fold and an overall yield of 21 %, using a two-step procedure, including ammonium sulfate precipitation and Q-sepharore chromatography. Molecular weight of the enzyme was 62.3 kDa according to SDS-PAGE data. The enzyme showed an optimum activity at pH 6.5 and 58 ˚C. Evolution of substrate specificity demonstrated that this thermostable enzyme had the highest activity towards para-nitrophenol acetate (pNPA, C2). Michaelis-Menten constant (Km) and maximum velocity (Vmax) of pNPA-hydrolyzing reaction were 12.6 mM and 550 U.mg-1, respectively. Enzyme activity was declined in the presence of metal ions (2 and 5 mM), including Fe2+, Ca2+, Cu2+, Zn2+, Mg2+ and Mn2+. The half-lives of purified esterase was 70 and 31 min at 60 °C and 80 °C, respectively. In conclusion, the enzyme is a novel thermostable lipolytic enzyme characterized from Shewanella species.

Keywords

Esterase; Thermostable; Lipolytic enzyme; Characterization;
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@article{paperid:1041845,
author = {Asoodeh, Ahmad and Ghorani Azam, Adel and ZARE ZARDINI, HADI},
title = {Biochemical Characterization of A Novel Thermophilic Esterase Isolated from Shewanella sp F88},
journal = {Journal of Sciences, Islamic Republic of Iran},
year = {2014},
volume = {25},
number = {1},
month = {June},
issn = {1016-1104},
pages = {5--12},
numpages = {7},
keywords = {Esterase; Thermostable; Lipolytic enzyme; Characterization; Shewanella},
}

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%0 Journal Article
%T Biochemical Characterization of A Novel Thermophilic Esterase Isolated from Shewanella sp F88
%A Asoodeh, Ahmad
%A Ghorani Azam, Adel
%A ZARE ZARDINI, HADI
%J Journal of Sciences, Islamic Republic of Iran
%@ 1016-1104
%D 2014

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