The 2nd Annual Congress of Stem Cells Research and Application , 2014-05-22

Title : ( Comparative analysis of the gene expression profile of chemokine receptors between adipose - derived and bone marrow - derived mesenchymal stem cells )

Authors: Hamid reza Bidkhori , Moein Farshchian , آسیه حیرانی , حجت نادری مشکین , Mahtab Dastpak , نغمه احمدیان کیا , Ahmad Reza Bahrami , Maryam Moghaddam Matin ,

Citation: BibTeX | EndNote

Introduction: Mesenchymal stem cells (MSCs) hold great promise in the field of regenerative medicine.Although originally isolated from bone marrow, MSCs have since been obtained from a variety of adult and neonatal tissues including the adipose tissue. Stemness and multipotential features of Mesenchymal Stem Cells (MSC) has been highlighted in many studies but there are many dark aspects in exclusive capabilities of MSC derived from different sources. Regarding adipose-derived MSCs, Ad-MSCs,and bone marrow-derived MSC, BM MSCs has been introduced and applicable in clinics, we designed a small molecule induction based approach for evaluation of gene expression response between these cells. Materials and Methods: A panel of MSC specific genes encoding CXCR4, CXCR6, CX3CR1 and CCR1 has been comparatively analyzed between Ad-MSCs and BM-MSCs by RealTime PCR, in response to hypoxia-mimicking agents CoCl2, DFX and VPA. Results: Our results showed CXCR4 expression dramatically increased following DFX and VPA treatment in Ad-MSC but decreased in BM MSCs, over expression of CCR1 was detected only in BM-MSCs but CXCR6 and CX3CR1didn't show any detectable expression between these kinds of MSCs. Conclusion: We speculate that differential expression profile of CXCR4 and CCR1 related to different capabilities of BM-MSCs and Ad-MSCs and further investigations necessary to determine which gene networks play the key roles in MSCs derived from different sources and these findings translated from bench to bed in novel cell therapies.

Keywords

, Adipose-derived MSCs, Bone marrow-derived MSCs, Chemokine Receptors, Gene
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@inproceedings{paperid:1042159,
author = {Bidkhori, Hamid Reza and Farshchian, Moein and آسیه حیرانی and حجت نادری مشکین and Dastpak, Mahtab and نغمه احمدیان کیا and Bahrami, Ahmad Reza and Moghaddam Matin, Maryam},
title = {Comparative analysis of the gene expression profile of chemokine receptors between adipose - derived and bone marrow - derived mesenchymal stem cells},
booktitle = {The 2nd Annual Congress of Stem Cells Research and Application},
year = {2014},
location = {مشهد, IRAN},
keywords = {Adipose-derived MSCs; Bone marrow-derived MSCs; Chemokine Receptors; Gene expression},
}

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%0 Conference Proceedings
%T Comparative analysis of the gene expression profile of chemokine receptors between adipose - derived and bone marrow - derived mesenchymal stem cells
%A Bidkhori, Hamid Reza
%A Farshchian, Moein
%A آسیه حیرانی
%A حجت نادری مشکین
%A Dastpak, Mahtab
%A نغمه احمدیان کیا
%A Bahrami, Ahmad Reza
%A Moghaddam Matin, Maryam
%J The 2nd Annual Congress of Stem Cells Research and Application
%D 2014

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