Title : ( Pretreatment of mesenchymal stem cells and stromal - derived factor - 1α delivery from chitosan - based injectable hydrogels for better cell guidance and retention )
Authors: حجت نادری مشکین , Maryam Moghaddam Matin , آسیه حیرانی , ملیحه حسن زاده , مینا شهریاری , نغمه احمدیان کیا , محمود رئیس المحدثین , ناصر سنجر موسوی , حمیدرضا بیدخوری , محمود حسینی , Ahmad Reza Bahrami ,
Full TextAbstract
Abstract: Clinical applications of mesenchymal stem cells (MSCs) rely on their capacity to home and engraft in the appropriate target tissues for a long time. Homing and engraftment capacity of these stem cells depend on the expression of Chemokines and their receptors. Ex vivo expanded MSCs exhibit homing potential when grafted to injury tissue but their homing efficiency has been observed very poor because of modifications in homing receptor expression and/or functions during culture and/or preparation steps. Hence, this study was designed to investigate the expression of surface CXCR4 by flow cytometric analysis (FACS) and in vitro modified Boyden chamber assay in adipose-derive MSCs (ASCs) stimulated with a hypoxia mimicking agents such as desferrioxamine mesilate (DFX), cobalt chloride (CoCl2), lithium chloride (LiCl), valproic acid (VPA) and hypoxia. Intracellular CXCR4 were also evaluated by conventional and real-time PCR. Then we evaluated the homing ability of DFX-pretreated human DiI-labeled ASCs
Keywords
, Chemotactic recruitment, Guided homing, Stem cells therapy
@inproceedings{paperid:1042160,
author = {حجت نادری مشکین and Moghaddam Matin, Maryam and آسیه حیرانی and ملیحه حسن زاده and مینا شهریاری and نغمه احمدیان کیا and محمود رئیس المحدثین and ناصر سنجر موسوی and حمیدرضا بیدخوری and محمود حسینی and Bahrami, Ahmad Reza},
title = {Pretreatment of mesenchymal stem cells and stromal - derived factor - 1α delivery from chitosan - based injectable hydrogels for better cell guidance and retention},
booktitle = {The 2nd Annual Congress of Stem Cells Research and Application},
year = {2014},
location = {مشهد, IRAN},
keywords = {Chemotactic recruitment، Guided homing، Stem cells therapy},
}
%0 Conference Proceedings
%T Pretreatment of mesenchymal stem cells and stromal - derived factor - 1α delivery from chitosan - based injectable hydrogels for better cell guidance and retention
%A حجت نادری مشکین
%A Moghaddam Matin, Maryam
%A آسیه حیرانی
%A ملیحه حسن زاده
%A مینا شهریاری
%A نغمه احمدیان کیا
%A محمود رئیس المحدثین
%A ناصر سنجر موسوی
%A حمیدرضا بیدخوری
%A محمود حسینی
%A Bahrami, Ahmad Reza
%J The 2nd Annual Congress of Stem Cells Research and Application
%D 2014
