Title : ( A fluorescent aptasensor for Potassium ion detection based triple-helix molecular switch )
Authors: asma verdian , Mohammad Reza Housaindokht , K. Abnous ,
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Abstract
Herein, a biosensor based on quadruplex-forming aptamer for the determination of K+ is presented. The aptamer was used as a molecular recognition element; it was adjacent to two arm fragments and a dual-labeled oligonucleotide serving as a signal transduction probe (STP) which is complementary of arm fragment sequence. In the presence of K+, the aptamer were displaced from STP, which was accompanied by decrease signal. The quenching percent of fluorescence intensity was proportional to the concentration of K+ in the range of 0.05 mM to 1.4 mM. A detection limit of 0.014 mM was achieved. Furthermore, other metal ions, such as Na+, Li+,NH4 +, Mg2+ and Ca2+ caused no notable interference on the detection of K+.
Keywords
, G-quadruplex, Aptamer, Potassium ion, Biosensor, Fluorescence aptasensor.
@article{paperid:1042765,
author = {Verdian, Asma and Housaindokht, Mohammad Reza and K. Abnous},
title = {A fluorescent aptasensor for Potassium ion detection based triple-helix molecular switch},
journal = {Analytical Biochemistry},
year = {2014},
volume = {466},
month = {August},
issn = {0003-2697},
pages = {72--75},
numpages = {3},
keywords = {G-quadruplex; Aptamer; Potassium ion; Biosensor; Fluorescence aptasensor.},
}
%0 Journal Article
%T A fluorescent aptasensor for Potassium ion detection based triple-helix molecular switch
%A Verdian, Asma
%A Housaindokht, Mohammad Reza
%A K. Abnous
%J Analytical Biochemistry
%@ 0003-2697
%D 2014
