Background: Antibiotic resistance is a serious and growing phenomenon in contemporary medicine and has emerged as one of the pre-eminent public health concerns of the 21st century. In this study, antibacterial activity of Mespilus germanica extract against some pathogenic bacterial strains (Streptococcus pyogene, Listeria innocua, Enterobacter aerogenes and Klebsiella pneumoniae) was evaluated.Materials and Methods: In this experimental study, maceration extraction method was used for Mespilus germanica extract. Disk diffusion method was used to evaluate the antimicrobial effect and broth microdilution method was used to determine the minimum inhibitory concentration and minimum bactericidal concentration. Then, the data were entered into the SPSS statistical software (v. 18) and analyzed using one-way ANOVA and Tukey’s test. Results: Antimicrobial activity was assessed by inhibition diameters which were found to range from 8–21.5 mm for the two extracts against all the bacterial strains tested. The Minimum Inhibitory Concentrations for the extracts were later determined by three fold serial dilutions method and they ranged from 2–64 mg/mL against all the strains and Minimum Bactericidal Concentrations for the extracts were later determined by three fold serial dilutions method and they ranged from 4–128 mg/mL against all the strains. Conclusion: The Mespilus germanica extract showed the more effective impact on the growth Streptococcus pyogene and Listeria innocua than Enterobacter aerogenes and Klebsiella pneumoniae (p<0.05). Mespilus germanica in comparison with common therapeutic antibiotics had more inhibitory effect on some of the studied strains in vitro.

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