International Journal of Peptide Research and Therapeutics, ( ISI ), Volume (20), No (4), Year (2014-10) , Pages (493-500)

Title : ( ACE-Inhibitory and Antioxidant Activityof Temporin-Ra Peptide: Biochemical Characterization and Molecular Modeling Study )

Authors: zahra mojallal taba tabaei , Ahmad Asoodeh , fateme asadi , hamid reza nezafati ,

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In this study, the inhibitory effect of Temporin-Ra (FP-14 peptide) on angiotensin converting enzyme(ACE) was evaluated. Inhibition mechanism was investi-gated by kinetic studies and molecular docking simulation.Lineweaver–Burk plot revealed that Temporin-Ra behavedas a non-competitive ACE inhibitor supported by the dock-ing simulation. The IC50 and Ki values were determined to be22.19 lM and 36 lg/ml, respectively. Molecular dockingsimulation showed that Temporin-Ra bound to both of N-and C-domains of ACE by forming hydrogen bonds andelectrostatic interactions; Temporin-Ra displayed higheraffinity to C-domain than N-domain. Antioxidant activity ofTemporin-Ra was examined using different methods. Theantioxidant activity of Temporin-Ra (0.2 mg/ml) in theinhibition of linoleic acid autoxidation was evaluated to be57 %. 1,1-diphenyl-2-picrylhydrazyl and 2, 2-azino-bis (3-ethylbenzothiazoline-6-sulphonicacid) diammonium saltradicals scavenging activities were 60 % at 0.5 mg/ml and37 % at 0.3 mg/ml, respectively. The hydroxyl radicalscavenging of FP-14 peptide at 0.33 mg/ml was 55 %. Theresults suggest that Temporin-Ra is a multifunctional peptidethat could be exploited to develop new anti-hypertensiondrugs and bio-compatible natural antioxidants.

Keywords

, Angiotensin I-converting enzyme, Antioxidant , Bioactive peptides, Molecular
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@article{paperid:1044013,
author = {Mojallal Taba Tabaei, Zahra and Asoodeh, Ahmad and Asadi, Fateme and Nezafati, Hamid Reza},
title = {ACE-Inhibitory and Antioxidant Activityof Temporin-Ra Peptide: Biochemical Characterization and Molecular Modeling Study},
journal = {International Journal of Peptide Research and Therapeutics},
year = {2014},
volume = {20},
number = {4},
month = {October},
issn = {1573-3149},
pages = {493--500},
numpages = {7},
keywords = {Angiotensin I-converting enzyme;Antioxidant ; Bioactive peptides; Molecular modeling},
}

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%0 Journal Article
%T ACE-Inhibitory and Antioxidant Activityof Temporin-Ra Peptide: Biochemical Characterization and Molecular Modeling Study
%A Mojallal Taba Tabaei, Zahra
%A Asoodeh, Ahmad
%A Asadi, Fateme
%A Nezafati, Hamid Reza
%J International Journal of Peptide Research and Therapeutics
%@ 1573-3149
%D 2014

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