The 2nd Annual Congress of Stem Cells Research and Application , 2014-05-22

Title : ( Genetic and Epigenetic landscape of Germline Stem Cells )

Authors: Moein Farshchian , Mahtab Dastpak , Maryam Moghaddam Matin , Dirk Geerts , Ahmad Reza Bahrami ,

Access to full-text not allowed by authors

Citation: BibTeX | EndNote

Elucidating the critical epigenetics events involved in differentiation and reprogramming of cells to primordial germ cells (PGCs) is among the interesting issues in stem cell research. Here, I will talk about critical transcription factors and global hypomethylation in development of germ cells. Evidence strongly suggests that the earliest PGCs emerging in the E7.25 mouse embryo epiblast have a highly methylated genome, and high level of H3K9me2 in chromatin but during development, genome demethylated and patterns of histone codes changes dramatically. We designed a polycistroniclentiviral vector and overexpressed Stella, Oct4 and Nanos2 simultaneously in transduced cells; Increasing level of Prdm14, Nanog and decreasing of G9a expression is an interesting finding which might be considered as a primary step of reprogramming toward germline progenitor cells, here we propose decreasing H3K9me2 level as a consequence of G9a down regulation is a critical step which facilitated transition to different stemness state through creating a new epigenetic memory for the early germ cells.

Keywords

, Epigenetic, hypomethylation, Germ line Stem Cells, polycistroniclentiviral
برای دانلود از شناسه و رمز عبور پرتال پویا استفاده کنید.

@inproceedings{paperid:1044373,
author = {Farshchian, Moein and Dastpak, Mahtab and Moghaddam Matin, Maryam and Dirk Geerts and Bahrami, Ahmad Reza},
title = {Genetic and Epigenetic landscape of Germline Stem Cells},
booktitle = {The 2nd Annual Congress of Stem Cells Research and Application},
year = {2014},
location = {مشهد, IRAN},
keywords = {Epigenetic; hypomethylation; Germ line Stem Cells; polycistroniclentiviral vector.},
}

[Download]

%0 Conference Proceedings
%T Genetic and Epigenetic landscape of Germline Stem Cells
%A Farshchian, Moein
%A Dastpak, Mahtab
%A Moghaddam Matin, Maryam
%A Dirk Geerts
%A Bahrami, Ahmad Reza
%J The 2nd Annual Congress of Stem Cells Research and Application
%D 2014

[Download]