EMBO Conference on Fission Yeast: pombe 2013 7 th International Fission Yeast Meeting , 2013-06-24

Title : ( The clathrin light chain is essential for cell wall synthesis and viability in Schizosaccharomyces pombe )

Authors: Nagore De León , Mirza Mohammad Reza Sharifmoghadam , Marta Hoya , M.- Ángeles Curto , Cristina Doncel , M.-Henar Valdivieso ,

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Abstract

The regulation of cell wall synthesis by the clathrin light chain has been addressed. Schizosaccharomyces pombe clc1Δ mutant was inviable in the absence of osmotic stabilization, while in other organisms as Saccharomyces cerevisiae and Dictyostelium discoideum clc1Δ mutants are viable in all the conditions tested. Why this difference is? When grown in sorbitol-supplemented medium clc1Δ cells grew slowly, formed aggregates, and had strong defects in morphology. Additionally, clc1Δ cells exhibited an altered cell wall composition. A mutant that allowed modulating the amount of Clc1p was created to analyze in more detail the dependence of cell wall synthesis on clathrin. A 40% reduction in the amount of Clc1p did not affect acid phosphatase secretion and bulk lipid internalization. Under these conditions, β(1,3)glucan synthase activity and cell wall synthesis were reduced. Also, the delivery of glucan synthases to the cell surface, and the secretion of the Eng1p glucanase were defective. These results suggest that the defects in the cell wall observed in the conditional mutant were due to a defective secretion of enzymes involved in the synthesis/remodeling of this structure, rather than to their endocytosis. Our results show that a reduction in the amount of clathrin that has minor effects on general vesicle trafficking has a strong impact on cell wall synthesis and perhaps, this is one of the main reasons why clc1 gen deletion is lethal in S. pombe.

Keywords

, clathrin, light chain, cell wall synthesis, Schizosaccharomyces pombe
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@inproceedings{paperid:1045323,
author = {Nagore De León and Sharifmoghadam, Mirza Mohammad Reza and Marta Hoya and M.- Ángeles Curto and Cristina Doncel and M.-Henar Valdivieso},
title = {The clathrin light chain is essential for cell wall synthesis and viability in Schizosaccharomyces pombe},
booktitle = {EMBO Conference on Fission Yeast: pombe 2013 7 th International Fission Yeast Meeting},
year = {2013},
location = {ENGLAND},
keywords = {clathrin; light chain; cell wall synthesis; Schizosaccharomyces pombe},
}

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%0 Conference Proceedings
%T The clathrin light chain is essential for cell wall synthesis and viability in Schizosaccharomyces pombe
%A Nagore De León
%A Sharifmoghadam, Mirza Mohammad Reza
%A Marta Hoya
%A M.- Ángeles Curto
%A Cristina Doncel
%A M.-Henar Valdivieso
%J EMBO Conference on Fission Yeast: pombe 2013 7 th International Fission Yeast Meeting
%D 2013

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