Purpose: :%!;3<'#) "/'/)="+>(%&"&)?: =@)$+&)A"/'8B)+<<#+C<'/)&C$%8+#&)"()!+(B)1'8/&D)$%A'E'#-)8"<<8')A%#F)"&)/%(')#'8+<'/)<%): =)7%#) retinopathy of prematurity (ROP). This paper introduces ROPAssist which is developed to enable automatic diagnosis of PLUS cases for ROP. A set of parameters are computed from the retinal image so that the software can be also used for research purposes. In order to provide ease of use, images are presented both as batch and individual forms. The software is also designed to be easy to learn and user friendly. Methods: ROPAssist is capable of computing tortuosity and vessel diameter in whole picture. An image bank, gathered by Farabi hospital in Tehran, is used to assess ROPAssist. The compendium consists of 48 images that are labeled as plus or non-plus independently by three experts. :'#<+"()"!+>');#%C'&&"(>)<'C$("G3'&)+#')+/%;<'/)<%)'H<#+C<)E'&&'8)<%#<3%&"<B)+(/)/"+!'<'#)7'+<3#'&D)<$')C8+&&"1C+<"%()/+<+)"&)&3,&'G3'(<8B)3&'/) <%)<#+"()%3#)/'C"&"%()&B&<'!)+(/)+)<$#'&$%8/)"&)<$'#')7%#)/'1('/)<%)"/'(<"7B);83&I(%(J;83&K) (%<$'#)%3<C%!')%7)<$')5L2 &&"&<)"&)<$')>'('#+<'/) vessel map which can be used for other applications as well. Results: CC%#/"(>)<%)'H;'#<&M)C%(&'(&3&)+(/)C8+&&"1C+<"%()#'&38<&-)5L2 &&"&<M&)9+;;+) "&)NKOPQN-)&'(&"<"E"<B) "&)NKRRSQ)+(/)&;'C"1C"<B) "&) NKPTUSK)V$')+8>%#"<$!)"&)8"+,8')<%)B"'8/)!%#')&">("1C+(<)#'&38<&)A"<$)"(C#'+&')"()5L2)"!+>')/+<+,+&')&"0'K Conclusion: ROPAssist can contribute in diagnosis of retinopathy of prematurity by reduction of subjectivity since it exhibits high correlation with experts’ consensus decisions. It also enhances the overall treatment time. As a result it can be used widely in clinical procedures

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