The 1st congress on Applied Zoology , 2015-01-28

Title : ( New Zealand rabbit’s blastema as a models for studing the interactions between amphibians and mammals tissues )

Authors: nasibeh mohammadzadeh , Maryam Moghaddam Matin , Nasser Mahdavi SHahri , Masoud Fereidoni ,

Citation: BibTeX | EndNote

Scaffolds constitute one of the components for tissue engineering applications, providing a three dimensional substrate to support cell growth and differentiation‌.Two effective factors on the mechanical properties of scaffolds are size and porosity. In this study, ECM derived from frog‟s lung (Pelophylax ridibundus) was used as a three dimensional environment to study the migration and polarity of cells originated from blastema tissue. In an experimental study, decellularization of the frog‟s lung was performed using physical (snap freeze -thaw) and chemical (SDS) methods. The prepared decellularized matrix was then assembled with the rings of the blastema tissue originated from pinnas of male New Zealand white rabbits (Oryctolagus cuniculus) and cultured for 30 days in vitro. The removal of the cells was confirmed by histotechniques. Inaddition, adhesion, polarity and migration of the blastema cells could be observed around the lung ECM took place. Thus, our results indicate that it is possible to prepare a natural 3D elastic scaffold from the frog‟s lung using SDS and blastema tissue has dynamic cells which could successfully migrate toward and into the scaffold. The New Zealand rabbit‟s blastema as a suitable models for studing the interactions with the amphibians‌ tissues.

Keywords

, animal model, decellularization, elastic scaffold, tissue engineering, blastema
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@inproceedings{paperid:1046532,
author = {Mohammadzadeh, Nasibeh and Moghaddam Matin, Maryam and Mahdavi SHahri, Nasser and Fereidoni, Masoud},
title = {New Zealand rabbit’s blastema as a models for studing the interactions between amphibians and mammals tissues},
booktitle = {The 1st congress on Applied Zoology},
year = {2015},
location = {مشهد, IRAN},
keywords = {animal model; decellularization; elastic scaffold; tissue engineering; blastema tissue},
}

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%0 Conference Proceedings
%T New Zealand rabbit’s blastema as a models for studing the interactions between amphibians and mammals tissues
%A Mohammadzadeh, Nasibeh
%A Moghaddam Matin, Maryam
%A Mahdavi SHahri, Nasser
%A Fereidoni, Masoud
%J The 1st congress on Applied Zoology
%D 2015

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