The aim of this study was to determine of the best size apex, media and plant growth regulators concentrations on the initiation of meristem tips from Sweet cherry trees cv. "Pishras-e- Mashhad". Ethanol and NaOCl were applied for disinfection. Meristems were isolated from actively growing plants in the spring and cultured on three kinds of basal media (MS, WPM and QL) supplement with three different concentrations of BA (0, 0.5 and 1 mgL-1), 0.1 mgL-1 GA3 and 0.1 mgL-1 IBA. Two apex sizes (0.2-0.4 and 0.5-0.7 mm) were used. Shoot tips were soaked in antioxidant solutions (100 mgL-1 ascorbic acid and 150 mgL-1 citric acid for 1 hours). Result showed that the highest survival rate and the low necrosis rate were in WPM medium supplemented with 1 mgL-1 BA (83.3%). The using of apex size in 0.5-0.7 mm and antioxidant solutions increased survival rate in meristems. Necrosis and contamination rate was high in MS (66.6%) and QL (36.1%) media without application of BA concentration, respectively.

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