Journal of Economic Entomology, Volume (108), No (3), Year (2015-5) , Pages (1-7)

Title : ( Application of the LAMP Assay as a Diagnostic Technique for Rapid Identification of Thrips tabaci (Thysanoptera: Thripidae) )

Authors: Lida Fekrat , Mohammad Zaki AGhl , vahid tahan ,

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Rapid and accurate identification of potentially invasive taxa that may cause high economic losses or environmental damage is of critical importance. The onion thrips, Thrips tabaci Lindeman, ranks as one of the world’s most destructive agricultural pests and commonly found in imported agriculturalmproducts and field samples, but is prone to undetected transport because of its minute size as well as cryptic behavior. Although traditional taxonomic methods are pretty useful in straightforward assignment of specimens to the genus Thrips, identification in the species level is much more difficult and requires expertise, knowledge, and experience. Furthermore, it is often difficult or impossible to identify or distinguish this species from other thrips by using material from other stages of development. Based on the foregoings, use of a molecular technique known as loop-mediated isothermal amplification (LAMP) as a rapid and robust alternative species diagnostic tool would be valuable. In this study, a relatively quick and simple method was used to detect the presence of onion thrips DNA rapidly and discriminate it from other species, by using material from different stages of development. Not only LAMP itself required less than 1 h to complete but also amounts of DNA as little as that recovered from a single specimen were adequate for the detection. Another advantage of this identification system is that nonspecialists will be able to make faster and cheaper identifications.

Keywords

, LAMP, loop-mediated isothermal amplification, molecular identification, rapid diagnostic tool, Thrips
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@article{paperid:1047792,
author = {Fekrat, Lida and Zaki AGhl, Mohammad and Tahan, Vahid},
title = {Application of the LAMP Assay as a Diagnostic Technique for Rapid Identification of Thrips tabaci (Thysanoptera: Thripidae)},
journal = {Journal of Economic Entomology},
year = {2015},
volume = {108},
number = {3},
month = {May},
issn = {0022-0493},
pages = {1--7},
numpages = {6},
keywords = {LAMP; loop-mediated isothermal amplification; molecular identification; rapid diagnostic tool; Thrips tabaci},
}

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%0 Journal Article
%T Application of the LAMP Assay as a Diagnostic Technique for Rapid Identification of Thrips tabaci (Thysanoptera: Thripidae)
%A Fekrat, Lida
%A Zaki AGhl, Mohammad
%A Tahan, Vahid
%J Journal of Economic Entomology
%@ 0022-0493
%D 2015

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