Journal of Cell and Molecular Research, Volume (7), No (1), Year (2015-9) , Pages (11-18)

Title : ( Cloning and Expression of Fusion (F) and Haemagglutinin-neuraminidase (HN) Epitopes in Hairy Roots of Tobacco (Nicotiana tabaccum) as a Step Toward Developing a Candidate Recombinant Vaccine Against Newcastle Disease )

Authors: Amir Ghaffar shahriari , Abdolreza Bagheri , Mohammad Reza Bassami , Saeid Malekzadeh Shafaroudi , Alireza Afsharifar ,

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Newcastle is a significant avian disease continuing to cause considerable loss. Developments in genetic engineering have led to plant-based platforms for human and animal vaccine production. Recombinant vaccine production in hairy root systems have several advantages over stable expression in whole plants, including high growth rates, ready genetic manipulations, high levels of recombinant protein production, and the potential for bioreactor culture. In an attempt to develop a recombinant vaccine in hairy roots, the sequences encoding fusion (F) and haemagglutinin-neuraminidase (HN) epitopes of Newcastle disease virus were cloned in pBI121 expression vector which was then transferred into leaf disks of tobacco (Nicotiana tabaccum) 'Turkish' cultivar by means of Agrobacterium rhizogenes. Hairy roots developed on MS medium containing 50 mg/L kanamycin and 30 mg/L meropenem. Incorporation of the heterologous gene in the genome of hairy roots was confirmed by PCR. Expression analyses were performed by real-time PCR at transcription level and by dot-blot and ELISA assays at translation level, all confirming the expression of the heterologous gene and production of the recombinant protein

Keywords

, Recombinant Vaccine, Antigen Expression, Newcastle Disease, Hairy Roots,
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@article{paperid:1048563,
author = {Shahriari, Amir Ghaffar and Bagheri, Abdolreza and Bassami, Mohammad Reza and Malekzadeh Shafaroudi, Saeid and Alireza Afsharifar},
title = {Cloning and Expression of Fusion (F) and Haemagglutinin-neuraminidase (HN) Epitopes in Hairy Roots of Tobacco (Nicotiana tabaccum) as a Step Toward Developing a Candidate Recombinant Vaccine Against Newcastle Disease},
journal = {Journal of Cell and Molecular Research},
year = {2015},
volume = {7},
number = {1},
month = {September},
issn = {2008-9147},
pages = {11--18},
numpages = {7},
keywords = {Recombinant Vaccine; Antigen Expression; Newcastle Disease; Hairy Roots; Agrobacterium Rhizogenes},
}

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%0 Journal Article
%T Cloning and Expression of Fusion (F) and Haemagglutinin-neuraminidase (HN) Epitopes in Hairy Roots of Tobacco (Nicotiana tabaccum) as a Step Toward Developing a Candidate Recombinant Vaccine Against Newcastle Disease
%A Shahriari, Amir Ghaffar
%A Bagheri, Abdolreza
%A Bassami, Mohammad Reza
%A Malekzadeh Shafaroudi, Saeid
%A Alireza Afsharifar
%J Journal of Cell and Molecular Research
%@ 2008-9147
%D 2015

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