Iranian Biomedical Journal, ( ISI ), Volume (20), No (1), Year (2016-1) , Pages (33-40)

Title : ( Modulation of Lipopolysaccharide Stimulated Nuclear Factor kappa B Mediated iNOS/NO Production by Bromelain in Rat Primary Microglial Cells )

Authors: soraya abbasi , Farzaneh Sabouni , Ali Moghimi , Saeed Ansari Majd ,

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Background: Microglial cells act as the sentinel of the central nervous system .They are involved in neuroprotection but are highly implicated in neurodegeneration of the aging brain. When over-activated, microglia release pro-inflammatory factors, such as nitric oxide (NO) and cytokines, which are critical in eliciting neuroinflammatory responses associated with neurodegenerative diseases. This study examined whether bromelain, the pineapple-derived extract, may exert an anti-inflammatory effect in primary microglia and may be neuroprotective by regulating microglial activation. Methods: Following the isolation of neonatal rat primary microglial cells, the activation profile of microglia was investigated by studying the effects of bromelain (5, 10, 20, and 30 µg/ml) on the levels of NO, inducible nitric oxide synthase (iNOS), and nuclear factor kappa B (NF-κB) in microglia treated with lipopolysaccharide (LPS) (1 µg/ml). Data were analyzed using Student's t-test. P values less than 0.05 were considered to be statistically significant, compared with the LPS-treated group without bromelain. Results: Results showed that pretreatment of rat primary microglia with bromelain, decreased the production of NO induced by LPS (1 µg/ml) treatment in a dose-dependent manner. Bromelain (30 µg/ml) also significantly reduced the expression of iNOS at mRNA level and NF-κB at protein level. Moreover, the study of mitochondrial activity in microglia indicated that bromelain had no cytotoxicity at any of the applied doses, suggesting that the anti-inflammatory effects of bromelain are not due to cell death. Conclusion: Bromelain can be of potential use as an agent for alleviation of symptoms in neurodegenerative diseases.

Keywords

, Microglia, Nitric oxide, NF-kappa B, Neuroimmunomodulation,
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@article{paperid:1050261,
author = {Abbasi, Soraya and Farzaneh Sabouni and Moghimi, Ali and Saeed Ansari Majd},
title = {Modulation of Lipopolysaccharide Stimulated Nuclear Factor kappa B Mediated iNOS/NO Production by Bromelain in Rat Primary Microglial Cells},
journal = {Iranian Biomedical Journal},
year = {2016},
volume = {20},
number = {1},
month = {January},
issn = {1028-852x},
pages = {33--40},
numpages = {7},
keywords = {Microglia; Nitric oxide; NF-kappa B; Neuroimmunomodulation; Ananas},
}

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%0 Journal Article
%T Modulation of Lipopolysaccharide Stimulated Nuclear Factor kappa B Mediated iNOS/NO Production by Bromelain in Rat Primary Microglial Cells
%A Abbasi, Soraya
%A Farzaneh Sabouni
%A Moghimi, Ali
%A Saeed Ansari Majd
%J Iranian Biomedical Journal
%@ 1028-852x
%D 2016

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