The 34th International Society of Animal Genetics Conference , 2014-07-27

Title : ( Widespread differential maternal and paternal genome effects on fetal bone phenotype at mid-gestation )

Authors: Ruidong Xiang , Alice Lee , Tanja Eindorf , Ali Javadmanesh , Madeleine Gugger , Zbigniew Kruk , Carolyn Fitzsimmons , Wayne Pitchford , Alison Leviton , Dana Thomsen , Gail Anderson , Ian Beckman , Brian Burns , David Rutley , Cory Xian , Stefan Hiendleder ,

Citation: BibTeX | EndNote

Abstract

Parent-of-origin dependent (epi)genetic factors are important determinants of prenatal development that program adult phenotype. However, data on magnitude and specificity of maternal and paternal genome effects on fetal bone are lacking. We used an outbred bovine model to dissect and quantify effects of parental genomes, fetal sex and non-genetic maternal effects on the fetal skeleton and analyzed phenotypic and molecular relationships between fetal muscle and bone. Analysis of 51 bone morphometric and weight parameters from 72 fetuses recovered at Day153 gestation (54% term) identified six principal components (PC1-6) that explained 80% of the variation in skeletal parameters. Parental genomes accounted for most of the variation in bone wet weight (PC1, 72.1%), limb ossification (PC2, 99.8%), flat bone size (PC4, 99.7%) and axial skeletal growth (PC5, 96.9%). Limb length showed lesser effects of parental genomes (PC3, 40.8%) and a significant non-genetic maternal effect (gestational weight gain, 29%). Fetal sex affected bone wet weight (PC1, P<0.0001) and limb length (PC3, P<0.05). Partitioning of variation explained by parental genomes revealed strong maternal genome effects on bone wet weight (74.1%, P<0.0001) and axial skeletal growth (93.5%,P<0.001), while paternal genome controlled limb ossification (95.1%, P<0.0001). Histomorphometric data revealed strong maternal genome effects on growth plate height (98.6%, P<0.0001) and trabecular thickness (85.5%, P<0.0001) in distal femur. Parental genome effects on fetal bone were mirrored by maternal genome effects on fetal serum 25-hydroxyvitamin D (96.9%, P<0.001) and paternal genome effects on alkaline phosphatase (90.0%, P<0.001) and their correlations with maternally controlled bone wet weight and paternally controlled limb ossification, respectively. Bone wet weight and flat bone size correlated positively with muscle weight (r=0.84 and 0.77, P<0.0001) and negatively with muscle H19 expression (r= 0.34 and 0.31, P<0.01). Since imprinted maternally expressed H19 regulates growth factors by miRNA interference, this suggests muscle-bone interaction via epigenetic factors.

Keywords

, Epigenetics, Maternal, Bovine fetus
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@inproceedings{paperid:1052858,
author = {Ruidong Xiang and Alice Lee and Tanja Eindorf and Javadmanesh, Ali and Madeleine Gugger and Zbigniew Kruk and Carolyn Fitzsimmons and Wayne Pitchford and Alison Leviton and Dana Thomsen and Gail Anderson and Ian Beckman and Brian Burns and David Rutley and Cory Xian and Stefan Hiendleder},
title = {Widespread differential maternal and paternal genome effects on fetal bone phenotype at mid-gestation},
booktitle = {The 34th International Society of Animal Genetics Conference},
year = {2014},
location = {Xian},
keywords = {Epigenetics; Maternal; Bovine fetus},
}

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%0 Conference Proceedings
%T Widespread differential maternal and paternal genome effects on fetal bone phenotype at mid-gestation
%A Ruidong Xiang
%A Alice Lee
%A Tanja Eindorf
%A Javadmanesh, Ali
%A Madeleine Gugger
%A Zbigniew Kruk
%A Carolyn Fitzsimmons
%A Wayne Pitchford
%A Alison Leviton
%A Dana Thomsen
%A Gail Anderson
%A Ian Beckman
%A Brian Burns
%A David Rutley
%A Cory Xian
%A Stefan Hiendleder
%J The 34th International Society of Animal Genetics Conference
%D 2014

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