Razavi International Journal of Medicine, Volume (3), No (4), Year (2015-12) , Pages (13-17)

Title : ( In Vitro Evaluation of the Effects of Zearalenone and α-Zearalenol on MCF-7 and MDA-MB-468 Cell Lines of Human Breast Cancer )

Authors: Rahmatollah parandin , Morteza Behnam Rassouli , Sajjad Sisakhtnezhad , Nasser Mahdavi SHahri ,

Access to full-text not allowed by authors

Citation: BibTeX | EndNote

Background: In the field of human breast cancer, the most recent researches referred to the influence of endogenous estrogen or exposure to environmental estrogen, as risk factors. Zearalenone (ZEN) as a mycotoxin and its derivative α- zearalenol (α-ZOL) are known nonsteroidal estrogenic compounds with potential endocrine disrupting properties. Objectives: The present study was designed to investigate the effects of ZEN and α-ZOL on human breast cancer cell lines MCF-7 and MDA-MB-468. Materials and Methods: Cell lines were treated by low and high doses of ZEN and α-ZOL (0, 1, 30, 62, 125, 250 and 500 ng/ml and 1, 2, 4, 8, 15, 30, 62 and 125 μg/ml) for 24 and 48 hours. Then MTT colorimetric assay was used to evaluate the cytotoxicity effect of ZEN and α-ZOL. Furthermore, morphological changes of treated and untreated cell lines were studied under an inverted microscope. Results: The results obtained from the present study demonstrated that both ZEN and α-ZOL enhance the cell viability of MCF-7 especially at low doses (1 - 500 ng/ml) and at a high dose of 125μg/ml after 24 and 48 hours. However, this effect for α-ZOL was somewhat greater than that for ZEN. On the other hand, these estrogenic compounds did not have any effect on the cell viability of MDA-MB-468. No morphological change was observed in treated cells. Conclusions: These results show that ZEN and α-ZOL enhance the rate of cell division in ER positive cells and therefore, exposure to this mycotoxin may increase the risk of breast cancer.

Keywords

, Zearalenone, Breast Cancer, MCF-7,
برای دانلود از شناسه و رمز عبور پرتال پویا استفاده کنید.

@article{paperid:1053042,
author = {Parandin, Rahmatollah and Behnam Rassouli, Morteza and Sajjad Sisakhtnezhad and Mahdavi SHahri, Nasser},
title = {In Vitro Evaluation of the Effects of Zearalenone and α-Zearalenol on MCF-7 and MDA-MB-468 Cell Lines of Human Breast Cancer},
journal = {Razavi International Journal of Medicine},
year = {2015},
volume = {3},
number = {4},
month = {December},
issn = {2345-6426},
pages = {13--17},
numpages = {4},
keywords = {Zearalenone; Breast Cancer; MCF-7; MDA-MB-468},
}

[Download]

%0 Journal Article
%T In Vitro Evaluation of the Effects of Zearalenone and α-Zearalenol on MCF-7 and MDA-MB-468 Cell Lines of Human Breast Cancer
%A Parandin, Rahmatollah
%A Behnam Rassouli, Morteza
%A Sajjad Sisakhtnezhad
%A Mahdavi SHahri, Nasser
%J Razavi International Journal of Medicine
%@ 2345-6426
%D 2015

[Download]