The 2nd congress of cytotechnology and its applications , 2015-10-20

Title : ( Use of ionic detergent SDS as a method for preparation of a natural scaffold from rabbit liver tissue )

Authors: Simin Ramezani , Maryam Moghaddam Matin , Nasser Mahdavi SHahri , sonia iran pour ,

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Introduction: Liver failure usually results from the inability of liver to perform its normal functions and leads to a high risk of mortality. Liver transplantation is currently recognized as a curative therapy for liver diseases but shortage of donor organs, high cost and immune rejection are major limitations of this technique. Hepatic tissue engineering can be considered as a good alternative for transplantation in liver patients. Threedimensional scaffolds play an important role in tissue engineering and improve cellular functions and formation of new tissues. Scaffolds derived from decellularized tissues with preservation of native extracellular matrix (ECM) are ideal sources for natural scaffolds. One important characteristic of scaffolds is porosity. In this regard, decellularized liver scaffold (DLS) contains many pores which allows proper diffusion of nutrient and oxygen which are important for cell growth. Methods: In this study, pieces of male liver tissue were prepared from New Zealand white rabbit and after washing with PBS, samples were maintained at -80°Cfor 24 h. Then, decellularization process was performed using treatments including of 12 and 24 h various incubation with 0.1% and 1% sodium dodecyl sulfate (SDS) solutions. Finally, in orderto evaluate of decellularization process and possible damageto the ECM, histological studies were carried out and samples were stained with hematoxylin-eosin, DAPI and picrofoshin. Results: Hematoxylin-eosin and DAPI staining reflected removal of nucleiand success of decellularization process with 0.1% SDS for 24 h. On the other hand, picrofoshin staining confirmed the preservation of collagen fibers in the scaffolds prepared by the above method. One of the main limiting factors in the advancement of liver tissue engineering is the lack of an ideal transplantable scaffold that supports cell attachment, proliferation, differentiation, and oxygen and nutrient transport. Thus, the ability to create a transplantable liver scaffold is an important advancement. In this study, treatment of liver pieces with 0.1% SDS solution successfully removed the cells without major effects on ECM components oftissue. More researches are needed to be done for a step in liver diseases therapy.

Keywords

, liver tissue, extracellular matrix, naturalscaffold, sodium dodecyl
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@inproceedings{paperid:1053819,
author = {Ramezani, Simin and Moghaddam Matin, Maryam and Mahdavi SHahri, Nasser and Iran Pour, Sonia},
title = {Use of ionic detergent SDS as a method for preparation of a natural scaffold from rabbit liver tissue},
booktitle = {The 2nd congress of cytotechnology and its applications},
year = {2015},
location = {مشهد, IRAN},
keywords = {liver tissue- extracellular matrix- naturalscaffold- sodium dodecyl sulfate},
}

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%0 Conference Proceedings
%T Use of ionic detergent SDS as a method for preparation of a natural scaffold from rabbit liver tissue
%A Ramezani, Simin
%A Moghaddam Matin, Maryam
%A Mahdavi SHahri, Nasser
%A Iran Pour, Sonia
%J The 2nd congress of cytotechnology and its applications
%D 2015

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