The 2nd congress of cytotechnology and its applications , 2015-10-20

Title : ( Studying the interactions between blastema tissue and three-dimensional matrix derived from rabbit liver in vitro )

Authors: Maryam Moghaddam Matin , Simin Ramezani , Nasser Mahdavi SHahri , sonia iran pour ,

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Introduction: Regeneration is a biological phenomenon which is widely observed in invertebrate species. vertebrates do not have such a remarkable ability but one of the best examples of healing process in mammals is the regeneration of damaged pinnas in rabbits that occurs by formation of blastema tissue on the periphery of the wound. Cells of blastema tissue have the ability of proliferation and differentiation similar to embryonic cells. Differentiate into specialized cell types is influenced by environmental factors, including extracellular matrix (ECM). ECM is a dynamic and complex environment that its interactions with cells can regulate behaviors such as cell migration, proliferationand differentiation. In this study, the interactions between blastema tissue and three-dimensional matrix derived from liver tissue were examined. Methods: In this study, liver tissue was obtained from male New Zealand white rabbits. For decellularization of the liver tissue, 0.1% sodium dodecyl sulfate (SDS) solution was used to remove cells from liver tissue. Then, decellularized matrices were assembled with the rings of the blastema tissues originated from pinnas of male New Zealand white rabbits and their interactions were studied using histological methods on days 5, 10, 15, 20, 25 and 30 in vitro. Results: Cell migration was not observed on the fifth day of culture, but blastema cells could be observed on the surface of scaffolds on tenth day. On the fifteenth and twentieth days, increased migration and penetration of cell into scaffolds was observed in the vascular areas of the liver scaffold. On the twenty-fifth day cell migration was reduced,so that no cells were observed within scaffold on the thirtieth day. Blastema tissue has dynamic cells which could successfully migrate into the scaffold and the natural three-dimensional scaffold prepared from rabbit liver could support survival, growth, and proliferation.The study showed that co-culture of blastema tissue and DCL is a suitable model for studying cellular behaviors such as migration and proliferation in vitro.

Keywords

, blastema tissue , extracellular matrix – interactions , decellularized
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@inproceedings{paperid:1053820,
author = {Moghaddam Matin, Maryam and Ramezani, Simin and Mahdavi SHahri, Nasser and Iran Pour, Sonia},
title = {Studying the interactions between blastema tissue and three-dimensional matrix derived from rabbit liver in vitro},
booktitle = {The 2nd congress of cytotechnology and its applications},
year = {2015},
location = {مشهد, IRAN},
keywords = {blastema tissue - extracellular matrix – interactions - decellularized liver},
}

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%0 Conference Proceedings
%T Studying the interactions between blastema tissue and three-dimensional matrix derived from rabbit liver in vitro
%A Moghaddam Matin, Maryam
%A Ramezani, Simin
%A Mahdavi SHahri, Nasser
%A Iran Pour, Sonia
%J The 2nd congress of cytotechnology and its applications
%D 2015

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