Title : ( In vitro histological investigation of interactions between rat decellularized large intestine scaffold and human adipose derived mesenchymal stem cells )
Authors: Somayeh Naderi , MaliheAkbarzadeh Niaki , Nasser Mahdavi SHahri , Maryam Moghaddam Matin , Masoud Fereidoni ,
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Abstract
Natural scaffolds derived from decellularized tissues and organs have been successfully used in tissue engineering.The aim of this study was to investigate the interactions between rat intestine decellularized scaffold and human adipose derived mesenchymal stem cells. Rat large intestine was dissected in fragments and decellularized by physicochemical methods. The scaffolds were loaded by human adipose derived mesenchymal stem cells expressing green fluorescent protein. Microscopic sections were prepared from the scaffolds after two weeks of culture with stem cells and studied by histological methods. The interactions of scaffolds with MSCs were also studied by electron microscopy. Histological and electron microscopy studies revealed human mesenchymal stem cell adhesion, migration, division and maintenance during the 14 days of culture in vitro. According to the results, scaffolds prepared from rat intestine matrix could be a suitable scaffold for studying in vitro cell behaviors such as division, migration and attachment. These various behaviors of cultured cells might be due to inductive effects of the extracellular matrix derived scaffold. However, more investigations are required to discover the exact effects of this scaffold and its interactions with mesenchymal stem cells.
Keywords
, scaffold , large intestine , mesenchymal stem cells, cell behaviors
@inproceedings{paperid:1053821,
author = {Somayeh Naderi and MaliheAkbarzadeh Niaki and Mahdavi SHahri, Nasser and Moghaddam Matin, Maryam and Fereidoni, Masoud},
title = {In vitro histological investigation of interactions between rat decellularized large intestine scaffold and human adipose derived mesenchymal stem cells},
booktitle = {The 2nd congress of cytotechnology and its applications},
year = {2015},
location = {مشهد, IRAN},
keywords = {scaffold - large intestine - mesenchymal stem cells- cell behaviors},
}
%0 Conference Proceedings
%T In vitro histological investigation of interactions between rat decellularized large intestine scaffold and human adipose derived mesenchymal stem cells
%A Somayeh Naderi
%A MaliheAkbarzadeh Niaki
%A Mahdavi SHahri, Nasser
%A Moghaddam Matin, Maryam
%A Fereidoni, Masoud
%J The 2nd congress of cytotechnology and its applications
%D 2015
