Yolk sac infection (YSI) and Dead-in-shell mortality caused by enterobactriacea in birds are not rare phenomenon, but there are only a few reports that show association between these conditions and Klebsiella spp. in canary chicks (Serinus canaria). High mortality in 1-3 days old canary chicks was reported in an indoor flock of canaries. In order to study the causative agent, yolk sac samples from Dead-in-shell and day old canary chicks were cultured. Klebsiella pneumonia was isolated and identified based on biochemical tests and using genus and species-specific multiplex PCR and then tested for their susceptibility to 15 antimicrobial agents. The isolates were showed susceptibility to Gentamycin, Chloramphenicol, Florfenicol and Streptomycin.

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