Background: Myiasis is defined as the infestation of live human and vertebrate animals with dipterous larvae for a certain period. There are reports indicating that dogs are the most common species affected by myiasis. This study was conducted to identify myiasis-causing flies in owned and stray dogs in Mashhad (Northeastern Iran). Methods: A total of 435 owned dogs and 800 stray dogs were examined for myiasis. Myiasis cases were cured and fly larvae were identified by microscopy using the relevant standard identification keys. Results: Ten out of 435 owned dogs (2.29 %) and 18 out of 800 stray dogs (2.25 %) had myiasis. The causative agents of myiasis in dogs based on their frequencies were as follows: Wohlfahrtia magnifica (50%), Lucilia sericata (28.57%) and Chrysomya albiceps (21.42%). Conclusion: W. magnifica was the most important myiasis-causing fly among the dogs sampled here, sometimes causing very serious damages. However, when treatment was given early enough, the larvae removed and the wound disinfected, the animals usually made a full recovery.

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