Plasmid, Volume (84), No (1), Year (2016-4) , Pages (51-60)

Title : ( Graphene oxide–cationic polymer conjugates: Synthesis and application as gene delivery vectors )

Authors: Mohsen Teimouri , azadeh hashem nia , Khalil Abnous , Hossein Eshghi , Mohammad Ramezani ,

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Nanomedicine as the interface between nanotechnology and medical sciences is a newarea that has attracted the attention of vast groups of researchers. Carbon nanomaterials are common platform for synthesis of nanoparticles for biomedical applications due to their low cytotoxicity and feasible internalization into mammalian cell lines (Yang et al., 2007; Arora et al., 2014; Oh and Park, 2014). Synthesis of vectors based on various cationic polymers polyethylenimine (PEI), polypropylenimine (PPI) and polyamidoamine (PAMAM) and their derivatives were considered as a strategy for transferring plasmid DNA and treatment of genetic diseases. Considering the lowcytotoxicity of graphene, chemicalmodification of its surface has led to fabrication of novel gene delivery systems based on graphene and graphene oxide. Hereinwe report the synthesis of three groups of vectors based on conjugation of graphene oxide (GO) with alkylated derivatives of three different cationic polymers (polyethylenimine (PEI), polypropylenimine (PPI) and polyamidoamine (PAMAM)) through different linkers including surface carboxyl group, glycine and spermidine. Two main challenges in design of gene delivery vectors is decreasing cytotoxicity while improving the transfection efficiency. All synthesized vectors showed significantly lower cellular toxicity compared to bare polymer. A plasmid encoding green fluorescent protein (GFP) was used to evaluate the transfection efficiency of nanoparticles both qualitatively using live cell fluorescent imaging and quantitatively using flow cytometry and each vectorwas compared to its polymer base. Most successful conjugation strategy was observed in the case of PEI conjugates among which most efficient vector was PEI–GO conjugate bearing glycine linker. This vector was 9 fold more effective in terms of the percent of EGFP transfected cells.

Keywords

, Graphene oxide, Functionalization, Polyethylenimine, Gene delivery, Polyamidoamine,
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@article{paperid:1056026,
author = {Teimouri, Mohsen and Hashem Nia, Azadeh and Khalil Abnous and Eshghi, Hossein and Mohammad Ramezani},
title = {Graphene oxide–cationic polymer conjugates: Synthesis and application as gene delivery vectors},
journal = {Plasmid},
year = {2016},
volume = {84},
number = {1},
month = {April},
issn = {0147-619X},
pages = {51--60},
numpages = {9},
keywords = {Graphene oxide; Functionalization; Polyethylenimine; Gene delivery; Polyamidoamine; Polypropylenimine},
}

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%0 Journal Article
%T Graphene oxide–cationic polymer conjugates: Synthesis and application as gene delivery vectors
%A Teimouri, Mohsen
%A Hashem Nia, Azadeh
%A Khalil Abnous
%A Eshghi, Hossein
%A Mohammad Ramezani
%J Plasmid
%@ 0147-619X
%D 2016

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