Title : ( Therapeutic effects of a combined antibiotic-enzyme treatment on subclinical mastitis in lactating dairy cows )
Authors: Babak KHoramian Toosi , M. Emaneini , M. Bolourchi , A. Niasari-Naslaji , Afsaneh Gorganzadeh , samira abani , P. Hovareshti ,Abstract
The objective of this study was to evaluate a combined antibiotic-enzyme therapy for Staphylococcus aureus mastitis and biofilm formation. A total of 141 cases of S. aureus chronic mastitis from three farms were divided into two groups: the control group (n = 54) were treated with Nafpenzal® ointment; the enzyme + antibiotic group (n = 87) were treated with Nafpenzal® plus an enzymatic ointment (MastiVeyxym®). Quantitative determination of biofilm formation was determined using a colorimetric microplate assay and the detection of ica genes by PCR. Enzyme + antibiotic therapy did not significantly improve cure rates compared to control (48.3 vs 38.9%). The cure rate for infections caused by biofilm-positive strains was 42.6 and 46.6% for control and enzyme + antibiotic groups, respectively (P > 0.05). Comparison of cure rates between farms showed a relationship with somatic cell count (SCC), parity and oxacillin resistance. 79.4% of the isolates produced biofilm and antibacterial resistance rates for oxacillin, penicillin and streptomycin were 25.5, 71.6 and 95.7%, respectively. These results indicate that while the increase in mastitis cure rate using enzymatic therapy was not significant, this treatment could be useful in some situations.
Keywords
, mastitis; Staphylococcus aureus; biofilm; enzyme, antibiotic treatment; genomic DNA; ica genes@article{paperid:1056634,
author = {KHoramian Toosi, Babak and M. Emaneini and M. Bolourchi and A. Niasari-Naslaji and Gorganzadeh, Afsaneh and Abani, Samira and P. Hovareshti},
title = {Therapeutic effects of a combined antibiotic-enzyme treatment on subclinical mastitis in lactating dairy cows},
journal = {Veterinarni Medicina},
year = {2016},
volume = {61},
number = {5},
month = {June},
issn = {0375-8427},
pages = {237--242},
numpages = {5},
keywords = {mastitis; Staphylococcus aureus; biofilm; enzyme-antibiotic treatment; genomic DNA; ica genes},
}
%0 Journal Article
%T Therapeutic effects of a combined antibiotic-enzyme treatment on subclinical mastitis in lactating dairy cows
%A KHoramian Toosi, Babak
%A M. Emaneini
%A M. Bolourchi
%A A. Niasari-Naslaji
%A Gorganzadeh, Afsaneh
%A Abani, Samira
%A P. Hovareshti
%J Veterinarni Medicina
%@ 0375-8427
%D 2016