International Journal of Biological Macromolecules, ( ISI ), Volume (94), No (1), Year (2017-1) , Pages (8270-835)

Title : ( Production and biochemical characterization of an alkaline protease from Aspergillus oryzae CH93 )

Authors: Ehsan Salehi , Ahmad Asoodeh , Mansour Aliabadian ,

Access to full-text not allowed by authors

Citation: BibTeX | EndNote

In this study, Aspergillus oryzae CH93 was isolated from soil sample and examined using molecular analysis. Following culture of A. oryzae CH93 under optimal enzyme production, a 47.5 kDa extracellular protease was purified using ammonium sulfate precipitation and Q-Sepharose chromatography. The optimal pH 8 and temperature of 50 °C obtained for the isolated protease. Sodium dodecyl sulfate (SDS), cetyltrimethyl ammonium bromide (CTAB), H2O2 decreased activity, while Triton X-100 and phenylmethanesulfonyl fluoride (PMSF) had no inhibitory effect on the enzyme activity; meanwhile, 2-mercaptoethanol and ethylenediaminetetraacetic acid (EDTA) declined the protease activity. Isoamyl alcohol and acetone (30%) enhanced activity whereas 2-propanol, isopropanol and dimethyl sulfoxide (DMSO) (30%) reduced protease activity. The enzyme exhibited a half-life of 100 min at its optimum temperature. Among five substrates of bovine serum albumin (BSA), N-acetyl-L-tyrosine ethyl ester monohydrate (ATEE), casein, azocasein and gelatin results showed that casein is the best substrate with Vmax of 0.1411 ± 0.004 μg/min and Km of 2.432 ± 0.266 μg/ml. In conclusion, the extracted protease from A. oryzae CH93 as a fungal source possessed biochemical features which could be useful in some application usages.

Keywords

Aspergillus. Alkaline protease. Enzyme kinetic.
برای دانلود از شناسه و رمز عبور پرتال پویا استفاده کنید.

@article{paperid:1056784,
author = {Salehi, Ehsan and Asoodeh, Ahmad and Aliabadian, Mansour},
title = {Production and biochemical characterization of an alkaline protease from Aspergillus oryzae CH93},
journal = {International Journal of Biological Macromolecules},
year = {2017},
volume = {94},
number = {1},
month = {January},
issn = {0141-8130},
pages = {8270--835},
numpages = {-7435},
keywords = {Aspergillus. Alkaline protease. Enzyme kinetic. Characterization},
}

[Download]

%0 Journal Article
%T Production and biochemical characterization of an alkaline protease from Aspergillus oryzae CH93
%A Salehi, Ehsan
%A Asoodeh, Ahmad
%A Aliabadian, Mansour
%J International Journal of Biological Macromolecules
%@ 0141-8130
%D 2017

[Download]