Journal of Food Processing and Preservation, ( ISI ), Volume (41), No (2), Year (2017-8) , Pages (1-8)

Title : ( Antioxidant and Angiotensin I Converting Enzyme (ACE) Inhibitory Properties of GL-9 Peptide )

Authors: hoda shabestarian , Ahmad Asoodeh , Masoud Homayouni-Tabrizi , هانیه حسین نژاداریانی ,

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The aim of this study was to examine antioxidant and inhibitory activity of Gly-Ala-Ser-Arg-His-Trp-Tyr-Phe-Leu peptide named GL-9 peptide. The synthetic GL-9 peptide was purified by C8 reveres phase HPLC (RP-HPLC). The antioxidant activity of the GL-9 peptide was investigated based on its scavenging capacity1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, 2,2′-azinobis (3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS), superoxide ( inline image), hydroxyl (OH•−) and lipid peroxidation inhibition. The ACE inhibitory activity and kinetic parameters of the peptide were determined using FAPPG as substrate. GL-9 peptide/ACE complex interaction, position, type and energy was also investigated by using molecular docking. GL-9 peptide displays DPPH (IC50 = 6.5 µg/mL), ABTS•+ (IC50 = 7.2 µg/mL), superoxide (IC50 = 122 µg/mL) and hydroxyl (IC50 = 60.0 µg/mL) radical scavengers. GL-9 peptide showed an IC50 value of 47.8 µg/mL which inhibited ACE activity. The results indicated that the GL-9 peptide has antioxidant and ACE inhibitory capacity. Practical Applications Synthetic GL-9 peptide antioxidant and anti-hypertensive activity were investigated using in vitro assays. Results indicated that this peptide possesses a high potent antioxidant activity as well as ACE-inhibitory activity, demonstrate its potential application as an ingredient in wholesome foodstuffs.

Keywords

, ANTIOXIDANT, ANGIOTENSIN I CONVERTING ENZYME (ACE) INHIBITORY, GL-9
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@article{paperid:1057200,
author = {Shabestarian, Hoda and Asoodeh, Ahmad and Masoud Homayouni-Tabrizi and هانیه حسین نژاداریانی},
title = {Antioxidant and Angiotensin I Converting Enzyme (ACE) Inhibitory Properties of GL-9 Peptide},
journal = {Journal of Food Processing and Preservation},
year = {2017},
volume = {41},
number = {2},
month = {August},
issn = {0145-8892},
pages = {1--8},
numpages = {7},
keywords = {ANTIOXIDANT; ANGIOTENSIN I CONVERTING ENZYME (ACE) INHIBITORY; GL-9 PEPTIDE},
}

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%0 Journal Article
%T Antioxidant and Angiotensin I Converting Enzyme (ACE) Inhibitory Properties of GL-9 Peptide
%A Shabestarian, Hoda
%A Asoodeh, Ahmad
%A Masoud Homayouni-Tabrizi
%A هانیه حسین نژاداریانی
%J Journal of Food Processing and Preservation
%@ 0145-8892
%D 2017

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