Introduction: Previous studies have shown anti-ulcerogenic effects of Artemisia sieberi (A. sieberi) on gastric lesions and experimental skin wound, so this study was conducted to evaluate healing effects of A. sieberi on the experimental second degree burn in mice skin. Methods: Ninety adult male mice in 3 groups were used. Second degree burn was made in the dorsum and then silver sulfadiazine (SSD) 1% ointment and A. sieberi extract were applied in the positive control and treatment groups respectively, while in the negative control no medication was done. Digital photographs were taken daily for determination of healing percentage. For histopathological assessment, 5 mice of each group on the days 1, 3, 7, 14 & 21 post burn, were chosen and after euthanasia, a full thickness skin flap of the burn was taken and after tissue processing, specimens were stained with Hematoxylin-Eosin (H&E) and examined for granulation tissue, inflammation, re-epithelialization and collagen sediment, also hydroxyproline content of burn was measured. Data were presented as mean ± SE and analyzed by using Kruskal-Wallis and Dunn post huc tests (P ≤ 0.05). Results: A. sieberi enhanced wound healing via significantly decreased inflammation, increased granulation tissue, hydroxyproline content and healing percentage in comparison to negative control in such a manner which was comparable to standard SSD. Conclusion: It seems that A. sieberi can promote burn healing due to anti-inflammatory, antioxidant, anti-microbial and mitogenic properties of the plant.

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