Molecular and Cellular Biochemistry, Volume (423), No (1), Year (2016-10) , Pages (141-149)

Title : ( GL-9 peptide regulates gene expression of CD44 cancer marker and pro-inflammatory cytokine TNF-α in human lung epithelial adenocarcinoma cell line (A549) )

Authors: Kosar Hooshmand , Ahmad Asoodeh , Fatemeh Behnam Rassouli ,

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In this study, we examined cytotoxic effect of GL-9 peptide on A459 cell line through studying the changes in TNF-a and CD44 gene expression and ROS production. Real-time PCR analysis showed that the treated A549 cells highly over expressed TNF-a, which was associated with a significant reduction of CD44 gene expression levels (p \ 0.05). ROS production rate was measured through the usage of DCFH-DA primer. Results demonstrated that GL-9 peptide could also induce cell death via ROS production. The effect of GL-9 peptide on human erythrocytes and leukocytes was analyzed. GL-9 peptide showed no significant toxic effect on human blood cells. Our results suggested that the GL-9 peptide as a potent natural agent could modulate gene expression of cancer cell markers.

Keywords

, Blood cells, Cell death, Peptide, Real-time polymerase chain reaction, Tumor necrosis
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@article{paperid:1058626,
author = {Hooshmand, Kosar and Asoodeh, Ahmad and Behnam Rassouli, Fatemeh},
title = {GL-9 peptide regulates gene expression of CD44 cancer marker and pro-inflammatory cytokine TNF-α in human lung epithelial adenocarcinoma cell line (A549)},
journal = {Molecular and Cellular Biochemistry},
year = {2016},
volume = {423},
number = {1},
month = {October},
issn = {0300-8177},
pages = {141--149},
numpages = {8},
keywords = {Blood cells; Cell death; Peptide; Real-time polymerase chain reaction; Tumor necrosis factor-alpha},
}

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%0 Journal Article
%T GL-9 peptide regulates gene expression of CD44 cancer marker and pro-inflammatory cytokine TNF-α in human lung epithelial adenocarcinoma cell line (A549)
%A Hooshmand, Kosar
%A Asoodeh, Ahmad
%A Behnam Rassouli, Fatemeh
%J Molecular and Cellular Biochemistry
%@ 0300-8177
%D 2016

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