Cellular and Molecular Biology, Volume (62), No (10), Year (2016-8) , Pages (26-31)

Title : ( RNAi-induced silencing in floral tissues of Petunia hybrida by agroinfiltration: a rapid assay for chalcone isomerase gene function analysis )

Authors: F. Keykha , Abdolreza Bagheri , Nasrin Moshtaghi , Ahmad Reza Bahrami , A. Sharifi ,

Citation: BibTeX | EndNote

Variegation in flower color is commonly observed in many plant species and also occurs on Petunia (Petunia hybrida) as an ornamental plant. Variegated plants are of highly valuable in the floricultural market. Agroinfiltration is an Agrobacterium-mediated transient assay for the analysis of gene function and genetic modification in leaves, flowers and fruit tissues of various plants. Transient RNAi-induced silencing by agroinfiltration has been developed in leaves and fruits of several plant species. Here we report the establishment of a transient hairpin RNAi-induced silencing system for color modification assay in floral tissues of Petunia with different colors. chiRNAi construct was cloned into the pBI121 vector under the control of 35S promoter. Transient RNA silencing of chi in the tissues of Petunia was induced by delivering 530 bp chi hairpin RNAs (hpRNAs) into the petals of using agroinfiltration. Impaired anthocyanin accumulation and reduction of endogenous mRNAs of the corresponding targets were observed in the infiltrated areas of the petals of four colors of Petunia. Silencing of the endogenous chi mRNAs was highly effective in reduction of chi gene and anthocyanin accumulation. This transient silencing system is a prototype for modification of the anthocyanin biosynthetic pathway in Petunia through chi gene suppression

Keywords

, Transient Transformation, Transient RNAi, Gene silencing, Anthocyanin degradation, Flower color,
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@article{paperid:1058747,
author = {F. Keykha and Bagheri, Abdolreza and Moshtaghi, Nasrin and Bahrami, Ahmad Reza and A. Sharifi},
title = {RNAi-induced silencing in floral tissues of Petunia hybrida by agroinfiltration: a rapid assay for chalcone isomerase gene function analysis},
journal = {Cellular and Molecular Biology},
year = {2016},
volume = {62},
number = {10},
month = {August},
issn = {0145-5680},
pages = {26--31},
numpages = {5},
keywords = {Transient Transformation; Transient RNAi; Gene silencing; Anthocyanin degradation; Flower color; Petunia.},
}

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%0 Journal Article
%T RNAi-induced silencing in floral tissues of Petunia hybrida by agroinfiltration: a rapid assay for chalcone isomerase gene function analysis
%A F. Keykha
%A Bagheri, Abdolreza
%A Moshtaghi, Nasrin
%A Bahrami, Ahmad Reza
%A A. Sharifi
%J Cellular and Molecular Biology
%@ 0145-5680
%D 2016

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