Progress in Biochemistry and Biophysics, Volume (43), No (9), Year (2016-7) , Pages (903-909)

Title : ( Anticancer effects of valproic acid on esophageal stem-like carcinoma cells )

Authors: saffiyeh saboormaleki , Fatemeh Behnam Rassouli , Maryam Moghaddam Matin , seyed mahdi moussavi ,

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Valproic acid (VPA) is a histone deacetylase inhibitor that has been an object of interest to clinicians for its promising potency in cancer therapy, as it induces apoptosis and differentiation, and enhances of chemotherapy sensitivity. Esophageal squamous cell carcinoma (ESCC) is a malignant disease with growing incidence and low survival rate. Due to limited information on VPA activity in ESCC cells, we aimed to determine effects of VPA on chemotherapy responsiveness and expression of malignant markers in ESCC stem-like cells. Upon coadministration of non-toxic VPA + cisplatin (DDP), paclitaxel and 5-fluorouracil, viability of KYSE30 cells was assessed, and induced apoptosis was evaluated by DAPI staining, DNA laddering and flow cytometry. In addition, real time RT-PCR was performed to study changes in the expression of P21, CD44 and BMI-1 upon treatments. MTT test demonstrated that VPA significantly (p<0.05) increased toxicity of DDP, which was confirmed by DNA laddering, flow cytometry analysis and significant (p<0.05) overexpression of P21. Moreover, real time RT-PCR results indicated significant (p<0.05) down regulation of CD44 and BMI-1 after VPA administration. Present attempt provided evidence, for the first time, that VPA not only improved responsiveness of esophageal stem-like cancer cells to DDP, also negatively regulated cancer stem cells markers in these cells.


, Valproic acid, Esophageal squamous cell carcinoma, Synergic activity
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author = {Saboormaleki, Saffiyeh and Behnam Rassouli, Fatemeh and Moghaddam Matin, Maryam and Moussavi, Seyed Mahdi},
title = {Anticancer effects of valproic acid on esophageal stem-like carcinoma cells},
journal = {Progress in Biochemistry and Biophysics},
year = {2016},
volume = {43},
number = {9},
month = {July},
issn = {1000-3282},
pages = {903--909},
numpages = {6},
keywords = {Valproic acid; Esophageal squamous cell carcinoma; Synergic activity},


%0 Journal Article
%T Anticancer effects of valproic acid on esophageal stem-like carcinoma cells
%A Saboormaleki, Saffiyeh
%A Behnam Rassouli, Fatemeh
%A Moghaddam Matin, Maryam
%A Moussavi, Seyed Mahdi
%J Progress in Biochemistry and Biophysics
%@ 1000-3282
%D 2016