Der Pharma Chemica, Volume (8), No (6), Year (2016-8) , Pages (16-22)

Title : ( Evaluation of vitamin E on microscopic parameters of chilled and frozen stored ram semen )

Authors: Mahnaz Ahmadi Hamedani , Abdol Mansour Tahmasbi , Abbas Ali Naserian , Yousef Jafari Ahangari ,

Citation: BibTeX | EndNote

The production of reactive oxygen species during the process of cryopreservation, decrease the motility and cellular viability by initiation of lipid per oxidation of bio-membranes. For reduce these damages, antioxidants are used as cryoprotectants against the lipid per oxidation. The aim of this study was to assess the influence of vitamin E adding to the dilution media on standard qualitative parameters (motility, progressive motility, viability, hypo-osmotic swelling test, Acrosomal damages and normal spermatozoa) of pre and post frozen-thawed ram semen. Semen samples were collected by Electro ejaculator from 6 Zel rams, and diluted with a Tris-base extender containing vitamin E (1, 2 and 3 m/M) and without antioxidants (control). Diluted semen was cooled to 5oC and frozen in 0.25 ml straw, prior to being stored in liquid nitrogen. The results show that vitamin E has positive protection effects on the semen characteristics in chilled and frozen thawed. Motility, progressive motility, viability, hypo-osmotic swelling test and total normality of spermatozoa were higher in all groups of vitamin E supplement compare with control group, but highest percentages of treats were observed in 2m/M of vitamin E. Also the effect of vitamin E on Acrosome damages were significant in post frozen conditions and the highest percentages were obtained in control group. Therefore we recommend using 2m/M of vitamin E in Tris extender for short and long storages of Zel ram spermatozoa.

Keywords

, Vitamin E, Zel ram,
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@article{paperid:1060021,
author = {Ahmadi Hamedani, Mahnaz and Tahmasbi, Abdol Mansour and Naserian, Abbas Ali and Yousef Jafari Ahangari},
title = {Evaluation of vitamin E on microscopic parameters of chilled and frozen stored ram semen},
journal = {Der Pharma Chemica},
year = {2016},
volume = {8},
number = {6},
month = {August},
issn = {0975-413x},
pages = {16--22},
numpages = {6},
keywords = {Vitamin E; Zel ram; Spermatozoa},
}

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%0 Journal Article
%T Evaluation of vitamin E on microscopic parameters of chilled and frozen stored ram semen
%A Ahmadi Hamedani, Mahnaz
%A Tahmasbi, Abdol Mansour
%A Naserian, Abbas Ali
%A Yousef Jafari Ahangari
%J Der Pharma Chemica
%@ 0975-413x
%D 2016

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