Journal of Equine Veterinary Science, ( ISI ), Volume (50), No (3), Year (2017-3) , Pages (44-51)

Title : ( Identification of Reliable Reference Genes for Quantitative Real-Time PCR in Equine Fibroblast-Like Synoviocytes Treated by Doxycycline )

Authors: samaneh ghasemi , pezhman Mirshokraei , H. Hassanpour , Kamran Sardari ,

Access to full-text not allowed by authors

Citation: BibTeX | EndNote

Real-time quantitative polymerase chain reaction is a powerful and accurate technique to evaluate gene transcription patterns in different cells under biological and pathological conditions. One of the critical steps in the quantitation of transcription profiles is accurate normalization. In this critical step, use of appropriate stable reference gene(s) would be important. The purpose of this study was to identify the most stable housekeeping genes in equine fibroblast-like synoviocytes (FLS) treated with doxycycline from a panel of candidate genes. The gene transcription levels of six commonly used reference genes (GAPDH, ACTB, PPIA, HPRT1, IPO8, and MRPL19) were determined in normal equine FLS or treated by doxycycline and the combination of both. After applying the BestKeeper, geNorm, and NormFinder programs, to this set of genes, there were differences in the ranked order in the three defined groups. But, overall resultants of all three analysis programs determined that the candidate reference genes could be ranked as HPRT1, ACTB, GAPDH, PPIA, MRPL19, and IPO8. To more accurate normalization of gene quantitative data, using of HPRT1, ACTB, and GAPDH combination as reference gene panels is recommended for equine FLS during normal condition or treated by doxycycline.

Keywords

, Fibroblast, like synoviocytes Housekeeping
برای دانلود از شناسه و رمز عبور پرتال پویا استفاده کنید.

@article{paperid:1060546,
author = {Ghasemi, Samaneh and Mirshokraei, Pezhman and H. Hassanpour and Sardari, Kamran},
title = {Identification of Reliable Reference Genes for Quantitative Real-Time PCR in Equine Fibroblast-Like Synoviocytes Treated by Doxycycline},
journal = {Journal of Equine Veterinary Science},
year = {2017},
volume = {50},
number = {3},
month = {March},
issn = {0737-0806},
pages = {44--51},
numpages = {7},
keywords = {Fibroblast-like synoviocytes Housekeeping gene Doxycycline Horse Stability},
}

[Download]

%0 Journal Article
%T Identification of Reliable Reference Genes for Quantitative Real-Time PCR in Equine Fibroblast-Like Synoviocytes Treated by Doxycycline
%A Ghasemi, Samaneh
%A Mirshokraei, Pezhman
%A H. Hassanpour
%A Sardari, Kamran
%J Journal of Equine Veterinary Science
%@ 0737-0806
%D 2017

[Download]