European Journal of Pharmaceutical Sciences, Volume (100), No (1), Year (2017-3) , Pages (176-186)

Title : ( The intracellular delivery of plasmid DNA using cationic reducible carbon nanotube — Disulfide conjugates of polyethylenimine )

Authors: azadeh hashem nia , Hossein Eshghi , Kalil Abnous , Mohammad Ramezani ,

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A series of polyethylenimine conjugates of single-walled carbon nanotube (PEI-SWNT) containing bioreducible disulfide bonds was synthesized and evaluated for their transfection efficiency. Different molecular weights of polyethylenimine (PEI) were thiolated with different mole ratio of 2-iminothiolane (2-IT). Single-walled carbon nanotube (SWNT) was first carboxylated and then three different cysteine-functionalized SWNT formulations were synthesized via introduced linkers: a) carbonyl group b) spermidine c) 1,8-diamino 3,6-dioxo octane. The final nanocarrierswere fabricated upon conjugation of thiolated PEIs and thiolatedSWNT via oxidative disulfide bond formation. All PEI-disulfide-SWNT conjugates were capable of DNA condensation and showed improved viability and transfection efficiency compared to PEI itself. Transfection efficiencies were up to 1500 times greater than PEI 25 kDa (C/P = 0.8). The results of this study suggest that the synthesized formulations based onSWNT-CO-Cysteine and PEI 1.8 kDawere themost efficient carriers. Considering the decreased cytotoxicity and higher transfection levels, the conjugates bear the potential for effective delivery of genetic materials.

Keywords

, Carbon nanotube, Disulfide, Bioreducible,
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@article{paperid:1061348,
author = {Hashem Nia, Azadeh and Eshghi, Hossein and Kalil Abnous and Mohammad Ramezani},
title = {The intracellular delivery of plasmid DNA using cationic reducible carbon nanotube — Disulfide conjugates of polyethylenimine},
journal = {European Journal of Pharmaceutical Sciences},
year = {2017},
volume = {100},
number = {1},
month = {March},
issn = {0928-0987},
pages = {176--186},
numpages = {10},
keywords = {Carbon nanotube; Disulfide; Bioreducible; Polyethylenimine},
}

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%0 Journal Article
%T The intracellular delivery of plasmid DNA using cationic reducible carbon nanotube — Disulfide conjugates of polyethylenimine
%A Hashem Nia, Azadeh
%A Eshghi, Hossein
%A Kalil Abnous
%A Mohammad Ramezani
%J European Journal of Pharmaceutical Sciences
%@ 0928-0987
%D 2017

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