Cancer Gene Therapy, Volume (24), No (4), Year (2017-4) , Pages (156-164)

Title : ( Promising gene delivery system based on polyethylenimine-modified silica nanoparticles )

Authors: Maryam Babaei , Hossein Eshghi , Kh Abnous , Mohammad Rahimizadeh , M Ramezani ,

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This article reports on the synthesis and full characterization of innovative silica-based nanoparticle composed of fumed silica as a core decorated with polyethylenimine (PEI) with different molecular weights (25, 10 and 1.8 kDa). Wide range of analytical, spectroscopic, and microscopic methods (TEM, DLS, ζ potential, elemental analysis (EA), TNBS and FTIR) were used to characterize the nanoparticles. Furthermore, transfection efficiency of these nanoparticles as non-viral vector was examined. The silica-PEI conjugates retained both the ability of PEI to fully condense plasmid DNA at low N/P ratios and suitable buffering capacity at the endosomal pH range. PEI-functionalized silica remarkably enhanced EGFP-N1 gene expression in murine neuroblastoma (Neuro-2A) cells up to 38 folds compared to PEI 25 kDa. Meanwhile the results of the cytotoxicity assays indicated that these silica-PEI conjugates have acceptable level of viability.

Keywords

, silica-based nanoparticle , polyethylenimine, transfection
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@article{paperid:1062433,
author = {Babaei, Maryam and Eshghi, Hossein and Kh Abnous and Rahimizadeh, Mohammad and M Ramezani},
title = {Promising gene delivery system based on polyethylenimine-modified silica nanoparticles},
journal = {Cancer Gene Therapy},
year = {2017},
volume = {24},
number = {4},
month = {April},
issn = {0929-1903},
pages = {156--164},
numpages = {8},
keywords = {silica-based nanoparticle ; polyethylenimine;transfection efficiency},
}

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%0 Journal Article
%T Promising gene delivery system based on polyethylenimine-modified silica nanoparticles
%A Babaei, Maryam
%A Eshghi, Hossein
%A Kh Abnous
%A Rahimizadeh, Mohammad
%A M Ramezani
%J Cancer Gene Therapy
%@ 0929-1903
%D 2017

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