Ultrasonic pre-treatment has been considered as an environmentally friendly process for enhancing the biodegradability of organic matter in anaerobic digestion. However the consumed energy during the pre-treatment is a matter of challenge especially where energy generation is the main purpose of a biogas plant. The aim of the present work was to study the efficiency of ultrasonic pre-treatment in enhancement of biogas production from fruits and vegetable wholesale market waste. Three sonication times (9, 18, 27 min) operating at 20 kHz and amplitude of 80 μm were used on the substrate. The highest methane yield was obtained at 18 min sonication (2380 kJ kg−1 total solids) while longer exposure to sonication led to lower methane yield. This amount of biogas was obtained in 12 d of batch time. The energy content of the biogas obtained from this reactor was two times of the input energy for sonication.

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