Cervical cancer is the fourth most common cancer among women, and the seventh overall most common cancer in the world. In cervical cancer, chemotherapy is considered as the first-line treatment. This procedure is effectively done with platinum complexes, although chemotherapy with cisplatin and its analogues still suffers from several drawbacks. As a result, it provides the impetus for the development of new metal complexes with better anticancer activity potential. In this study, we investigated the cytotoxic effects and anticancer activity of Ag pyridine complexon cervical cancer (HeLa) and normal (HDF) cell lines for the fist time.To do so, cells were treated with various concentrations of this compound for 24, 48 and 72 hours. Then, cell viability was evaluated by MTT assay. The results of the MTT assay showed that pyridinedicarboxylato Ag(I)complex had cytotoxic effects on HeLa cells with IC50 value of about 5μg/ml after 48 hours of treatment. Moreover, IC50 value of cisplatin was approximately 2μg/ml after 48 hours of treatment. In addition, the results indicated thatpyridinedicarboxylato Ag(I)complex had lower cytotoxic effects on normal HDF cells with IC50 values of about 13, 17 and 10μg/ml after 24, 48 and 72 hours of treatment, respectively. The results indicate that understanding of structure–activity relationship is considered as a prospective investigation so as to find a more effective drug compered to cisplatin.

کلمات کلیدی