In this work, the multifunctional temperature-sensitive magnetoliposomal nanostructures are designed and fabricated by investigating the effects of numerous parameters such as buffers types, concentration, pH amounts as well as liposomes compositions, and features of conjugated magnetic nanoparticles. In order to analyze different properties of synthesized nanoparticles and fabricated liposomes in each step, various techniques such as XRD, Raman spectroscopy, VSM, TEM, DLS, ICP-OES, and AFM were employed. Based on results, the stability of Fe3O4 nanoparticles in citrate buffer is more appropriate compared to phosphate buffer. Also, the most appropriate amount of pH buffer is equal to 6.5 for desired loading of nanoparticles while the most suitable composition consisting of 86 wt% DPPC, 10 wt% Cholesterol, and 4 wt% m-PEG2000-DSPE, with loading percentage of about 39%. Accordingly, it can be concluded that quality of nanoparticles loading depends on the different parameters such as buffer type, buffer concentration, buffer pH amount, liposomes compositions, and stoichiometries of their components. Also, based on cytotoxicity analyses on the C26 colon carcinoma cells, after 48 h cell culture treatment, the IC50 values of pure magnetic nanoparticles and liposomal encapsulated nanoparticles are equal to 41 and 19%, respectively which show no cytotoxicity in these analyses and improve biocompatibility by using liposomal capsulation system.

Keywords