Title : ( A sensitive electrochemical aptasensor based on single wall carbon nanotube modified screen printed electrode for detection of Escherichia coli O157:H7 )
Authors: Mohammad Reza Housaindokht , asma verdian , Elham Sheikhzadeh , parvaneh pordeli , Zeinab Rouhbakhsh Zaeri , fatemeh janati fard , MASOUMEH NOSRATI , Mansour Mashreghi , Alireza Haghparast , Ali Nakhaei Pour , Abbas Ali Esmaeili , safoura soleymani ,
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Abstract
Escherichia coli O157:H7 (E.coli O157:H7), is an extremely infectious and potentially fatal water and food-borne pathogen, responsible for numerous hospitalisations and deaths all over the world. Herein we reported the development of simple and label-free electrochemical aptasensor based on single wall carbon nanotube (SWCNT) modified screen printed electrode (SPE) to detect E.coli O157:H7 bacteria. The electrochemical probe of methylene blue (MB) was used to investigate the interactions between aptamer and E.coli O157:H7. The resulting MB/Apt/SWNT/SPE layer exhibited good current response to detect E.coli O157:H7. The aptasensor detect E.coli O157:H7 in the concentration range of 1.7 × 101 - 1.1 × 107 CFU mL-1 and limit of quantification of 1.7 × 101 CFU mL-1. The proposed aptasensor is rapid, convenient and low-cost for effective sensing of E.coli O157:H7 with high selectivity over interference bacteria. The suitability of the aptasensor for real sample measurements was investigated by recovery studies in tap water sample.
Keywords
, Escherichia coli O157:H7, aptamer, electrochemical aptasensor, label-free detection.
@article{paperid:1065515,
author = {Housaindokht, Mohammad Reza and Verdian, Asma and Sheikhzadeh, Elham and Pordeli, Parvaneh and Rouhbakhsh Zaeri, Zeinab and Janati Fard, Fatemeh and NOSRATI, MASOUMEH and Mashreghi, Mansour and Haghparast, Alireza and Nakhaei Pour, Ali and Esmaeili, Abbas Ali and Soleymani, Safoura},
title = {A sensitive electrochemical aptasensor based on single wall carbon nanotube modified screen printed electrode for detection of Escherichia coli O157:H7},
journal = {Advanced Materials Letters},
year = {2018},
month = {March},
issn = {0976-3961},
keywords = {Escherichia coli O157:H7; aptamer; electrochemical aptasensor; label-free detection.},
}
%0 Journal Article
%T A sensitive electrochemical aptasensor based on single wall carbon nanotube modified screen printed electrode for detection of Escherichia coli O157:H7
%A Housaindokht, Mohammad Reza
%A Verdian, Asma
%A Sheikhzadeh, Elham
%A Pordeli, Parvaneh
%A Rouhbakhsh Zaeri, Zeinab
%A Janati Fard, Fatemeh
%A NOSRATI, MASOUMEH
%A Mashreghi, Mansour
%A Haghparast, Alireza
%A Nakhaei Pour, Ali
%A Esmaeili, Abbas Ali
%A Soleymani, Safoura
%J Advanced Materials Letters
%@ 0976-3961
%D 2018
