Cell and Tissue Banking, ( ISI ), Volume (18), No (4), Year (2017-12) , Pages (489-500)

Title : ( L )

Authors: Shirin Toosi , Hojjat Naderi Meshkin , Fatemeh Kalalinia , Mohammad Taghi Pievandi , Hossein Hosseinkhani , Ahmad Reza Bahrami , Asieh Heirani-Tabasi , Mahdi Mirahmadi , Javad Behravan ,

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Abstract

Mesenchymal stem cells (MSCs) have been designated as the most reliable cells in clinics to treat osteo-diseases because of their versatile nature. MSCs, isolated from long bone (Lb-MSCs) are rarely reported and named as RIA-MSCs because of the reamer–irrigator–aspirator (RIA) device. The potential of these cells in the treatment of non-union bone fractures made them the ideal candidates to be studied for clinical practices. In this work, effect of cryopreservation on the proliferation and differentiation capabilities of long bone MSCs (Lb-MSCs) has been studied. For this purpose, Lb-MSCs were isolated via RIA device and characterized using flow cytometry and differentiation assays. Cells were cryopreserved for 3, 6 and 12 months and thereafter were characterized using differentiation assays and genetic markers specific for osteogenic, chondrogenic, and adipogenic potential quantitatively by qRT-PCR. Lb-MSCs were found expressing MSC characteristic markers defining their identity. The population doubling time (PDT) was about 2.5 ± 0.5 days and colonies appeared after 7–10 days. Differentiation potential and gene expression of 3, 6 and 12 months cryopreserved Lb-MSCs were unaltered. The results show that cryopreservation did not have an effect on the differentiation potential of human Lb-MSCs. Therefore, our work offers Lb-MSCs as clinically cells for treating osteo-diseases.

Keywords

, Long bone, Mesenchymal stem cells, Cryopreservation, Differentiation, Cell banking, Stem cell therapy
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@article{paperid:1065868,
author = {Shirin Toosi and Hojjat Naderi Meshkin and Fatemeh Kalalinia and Mohammad Taghi Pievandi and Hossein Hosseinkhani and Bahrami, Ahmad Reza and Asieh Heirani-Tabasi and Mahdi Mirahmadi and Javad Behravan},
title = {L},
journal = {Cell and Tissue Banking},
year = {2017},
volume = {18},
number = {4},
month = {December},
issn = {1389-9333},
pages = {489--500},
numpages = {11},
keywords = {Long bone; Mesenchymal stem cells; Cryopreservation; Differentiation; Cell banking; Stem cell therapy},
}

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%0 Journal Article
%T L
%A Shirin Toosi
%A Hojjat Naderi Meshkin
%A Fatemeh Kalalinia
%A Mohammad Taghi Pievandi
%A Hossein Hosseinkhani
%A Bahrami, Ahmad Reza
%A Asieh Heirani-Tabasi
%A Mahdi Mirahmadi
%A Javad Behravan
%J Cell and Tissue Banking
%@ 1389-9333
%D 2017

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