گیاهان دارویی- Journal of Medicinal Plants, Volume (17), No (65), Year (2018-10) , Pages (73-82)

Title : ( Effective Induction of Hairy Roots in Persian Poppy (Papaver bracteatum Lindl.) Using Sonication Method )

Authors: Majid Azizi ,

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Abstract

Background: Papaver bracteatum Lindl. Commonly known as Iranian poppy is an important medicinal plant due to the presence of benzylisoquinoline alkaloids. Objective: To evaluate the hairy root culture as a novel method for thebaine production. Methods: To optimize the hairy root culture of P. bracteatum, five strains of Agrobacterium rhyzogenes (ATCC15834, C318, A13, 9453 and A4) were used by means of sonication-assisted Agrobacterium-mediated transformation (SAAT). Results: Strain C318 proved to be more efficient than the other strains to hairy root induction ability. Among different experimental conditions, the highest transformation rate (28.5 %) was observed 4 weeks after inoculation, once the explants were subjected to sonication (45 s) following a heat treatment of 40°C for 5 min. Transgenic status of the hairy roots was confirmed by PCR using rolB-specific primers. Conclusion: HPLC analysis showed the ability of hairy roots to synthesize of thebaine as a valuable medicinal compound.

Keywords

, Papaver bracteatum Lindl., Hairy root, Sonication-assisted Agrobacterium-mediated transformation, Thebaine
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@article{paperid:1066806,
author = {Azizi, Majid},
title = {Effective Induction of Hairy Roots in Persian Poppy (Papaver bracteatum Lindl.) Using Sonication Method},
journal = {گیاهان دارویی- Journal of Medicinal Plants},
year = {2018},
volume = {17},
number = {65},
month = {October},
issn = {1684-0240},
pages = {73--82},
numpages = {9},
keywords = {Papaver bracteatum Lindl.; Hairy root; Sonication-assisted Agrobacterium-mediated transformation; Thebaine},
}

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%0 Journal Article
%T Effective Induction of Hairy Roots in Persian Poppy (Papaver bracteatum Lindl.) Using Sonication Method
%A Azizi, Majid
%J گیاهان دارویی- Journal of Medicinal Plants
%@ 1684-0240
%D 2018

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