Ibuprofen, a non-steroidal anti-inflammatory drug (NSAIDs), blocks action of both COX1 and COX2 enzymes. Recently, some new aspect of pharmacological activity of ibuprofen other than the conventional known activity as NSAIDs has been discovered, including protection against neurodegenerative disorders.In this study we have evaluated the effects of ibuprofen on cell death induced by 6-hydroxydopamine in PC12 cells in the mimicry of Parkinson disease by measurement of cell viability, the amount of reactive oxygen species (ROS) and apoptosis. According to the results, pre-treatment with ibuprofen significantly increased the amount of cell viability (p<0.001), decreased the amount of ROS (p<0.001) and apoptosis (p<0.001) in PC12 cells adjacent with 6-hydroxydopamine. As a result, probably pre-treatment with ibuprofen by scavenging ROS and inducing antioxidant effect could potentially result in a protection against the 6-hydroxydopamine effects on dopaminergic cells. Overall, it is suggested that ibuprofen may be able to produce anti-oxidant effects by inhibiting the COX1 and COX2 enzymes.

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